Technical and statistical improvements for flow cytometric DNA analysis of paraffin‐embedded tissue

Brenda J. Sickle‐Santanello, William B. Farrar, Jane F. Decenzo, Sedigheh Keyhani‐Rofagha, John Klein, Dennis Pearl, Harry Laufman, Robert V. O'Toole

Research output: Contribution to journalArticle

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Abstract

Flow cytometric DNA analysis of paraffinembedded solid tumors has permitted review of large series of archival tissue in attempts to relate abnormal DNA content to prognosis. Limitations of the technique include: (1) a laborious, time‐consuming procedure; (2) variation in technique between laboratories; and (3) lack of an objective method of computing DNA indices. Critical evaluaiton of our technique has shortened the time involved in dewaxing and rehydration, selectively utilized patient's own normal tissue as the internal standard, proved reproducibility of stored specimens, standardized DNA index computation, and developed a statistical analysis to confirm aneuploidy. These technical improvements and the development of a statistical analysis provide a way to shorten the procedure time and standardize the data generated from flow cytometric DNA analysis so as to improve the quality of retrospective reviews of paraffin‐embedded tumors and accelerate the definition of flow cytometry's role as a prognostic indicator.

Original languageEnglish (US)
Pages (from-to)594-599
Number of pages6
JournalCytometry
Volume9
Issue number6
DOIs
StatePublished - Nov 1988

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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    Sickle‐Santanello, B. J., Farrar, W. B., Decenzo, J. F., Keyhani‐Rofagha, S., Klein, J., Pearl, D., Laufman, H., & O'Toole, R. V. (1988). Technical and statistical improvements for flow cytometric DNA analysis of paraffin‐embedded tissue. Cytometry, 9(6), 594-599. https://doi.org/10.1002/cyto.990090613