Telomerase activity in the normal and neoplastic rat mammary gland

Debora Varon, Cheng Jiang, Catigan Hedican, Jeffrey S. Dome, Christopher B. Umbricht, Lisa A. Carey, Henry J. Thompson, Saraswati Sukumar

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The 1-methyl-1-nitrosourea-induced rat mammary tumor model system is well studied, reproducible, and widely used. We have investigated whether these tumors possess higher telomerase activity than normal mammary tissue. Using the telomeric repeat amplification protocol assay, we found significantly higher telomerase activity in 36 mammary carcinomas than in 72 mammary glands of virgin rats. The level of telomerase activity in virgin rats was unaffected by strain, age, stage of the estrous cycle, or ovariectomy. However, mammary glands obtained from pregnant rats exhibited telomerase activity comparable to that found in the tumors, possibly reflecting the high epithelial content of these tissues. Indeed, isolated epithelial cells from virgin and pregnant mammary glands and from carcinomas had similar telomerase activities. Thus, telomerase activity is constitutive in the rat mammary epithelium and is not a unique characteristic of malignant transformation in this tissue. These results underscore the importance of attributing biochemical properties to specific cell types in a tissue, a situation not paralleled in the interpretation of data from in vitro models.

Original languageEnglish (US)
Pages (from-to)5605-5609
Number of pages5
JournalCancer Research
Volume57
Issue number24
StatePublished - Dec 15 1997

Fingerprint

Telomerase
Human Mammary Glands
Breast Neoplasms
Breast
Epithelium
Estrous Cycle
Ovariectomy
Neoplasms
Epithelial Cells

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Varon, D., Jiang, C., Hedican, C., Dome, J. S., Umbricht, C. B., Carey, L. A., ... Sukumar, S. (1997). Telomerase activity in the normal and neoplastic rat mammary gland. Cancer Research, 57(24), 5605-5609.
Varon, Debora ; Jiang, Cheng ; Hedican, Catigan ; Dome, Jeffrey S. ; Umbricht, Christopher B. ; Carey, Lisa A. ; Thompson, Henry J. ; Sukumar, Saraswati. / Telomerase activity in the normal and neoplastic rat mammary gland. In: Cancer Research. 1997 ; Vol. 57, No. 24. pp. 5605-5609.
@article{219dba530bd847ba84c86c413507958a,
title = "Telomerase activity in the normal and neoplastic rat mammary gland",
abstract = "The 1-methyl-1-nitrosourea-induced rat mammary tumor model system is well studied, reproducible, and widely used. We have investigated whether these tumors possess higher telomerase activity than normal mammary tissue. Using the telomeric repeat amplification protocol assay, we found significantly higher telomerase activity in 36 mammary carcinomas than in 72 mammary glands of virgin rats. The level of telomerase activity in virgin rats was unaffected by strain, age, stage of the estrous cycle, or ovariectomy. However, mammary glands obtained from pregnant rats exhibited telomerase activity comparable to that found in the tumors, possibly reflecting the high epithelial content of these tissues. Indeed, isolated epithelial cells from virgin and pregnant mammary glands and from carcinomas had similar telomerase activities. Thus, telomerase activity is constitutive in the rat mammary epithelium and is not a unique characteristic of malignant transformation in this tissue. These results underscore the importance of attributing biochemical properties to specific cell types in a tissue, a situation not paralleled in the interpretation of data from in vitro models.",
author = "Debora Varon and Cheng Jiang and Catigan Hedican and Dome, {Jeffrey S.} and Umbricht, {Christopher B.} and Carey, {Lisa A.} and Thompson, {Henry J.} and Saraswati Sukumar",
year = "1997",
month = "12",
day = "15",
language = "English (US)",
volume = "57",
pages = "5605--5609",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "24",

}

Varon, D, Jiang, C, Hedican, C, Dome, JS, Umbricht, CB, Carey, LA, Thompson, HJ & Sukumar, S 1997, 'Telomerase activity in the normal and neoplastic rat mammary gland', Cancer Research, vol. 57, no. 24, pp. 5605-5609.

Telomerase activity in the normal and neoplastic rat mammary gland. / Varon, Debora; Jiang, Cheng; Hedican, Catigan; Dome, Jeffrey S.; Umbricht, Christopher B.; Carey, Lisa A.; Thompson, Henry J.; Sukumar, Saraswati.

In: Cancer Research, Vol. 57, No. 24, 15.12.1997, p. 5605-5609.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Telomerase activity in the normal and neoplastic rat mammary gland

AU - Varon, Debora

AU - Jiang, Cheng

AU - Hedican, Catigan

AU - Dome, Jeffrey S.

AU - Umbricht, Christopher B.

AU - Carey, Lisa A.

AU - Thompson, Henry J.

AU - Sukumar, Saraswati

PY - 1997/12/15

Y1 - 1997/12/15

N2 - The 1-methyl-1-nitrosourea-induced rat mammary tumor model system is well studied, reproducible, and widely used. We have investigated whether these tumors possess higher telomerase activity than normal mammary tissue. Using the telomeric repeat amplification protocol assay, we found significantly higher telomerase activity in 36 mammary carcinomas than in 72 mammary glands of virgin rats. The level of telomerase activity in virgin rats was unaffected by strain, age, stage of the estrous cycle, or ovariectomy. However, mammary glands obtained from pregnant rats exhibited telomerase activity comparable to that found in the tumors, possibly reflecting the high epithelial content of these tissues. Indeed, isolated epithelial cells from virgin and pregnant mammary glands and from carcinomas had similar telomerase activities. Thus, telomerase activity is constitutive in the rat mammary epithelium and is not a unique characteristic of malignant transformation in this tissue. These results underscore the importance of attributing biochemical properties to specific cell types in a tissue, a situation not paralleled in the interpretation of data from in vitro models.

AB - The 1-methyl-1-nitrosourea-induced rat mammary tumor model system is well studied, reproducible, and widely used. We have investigated whether these tumors possess higher telomerase activity than normal mammary tissue. Using the telomeric repeat amplification protocol assay, we found significantly higher telomerase activity in 36 mammary carcinomas than in 72 mammary glands of virgin rats. The level of telomerase activity in virgin rats was unaffected by strain, age, stage of the estrous cycle, or ovariectomy. However, mammary glands obtained from pregnant rats exhibited telomerase activity comparable to that found in the tumors, possibly reflecting the high epithelial content of these tissues. Indeed, isolated epithelial cells from virgin and pregnant mammary glands and from carcinomas had similar telomerase activities. Thus, telomerase activity is constitutive in the rat mammary epithelium and is not a unique characteristic of malignant transformation in this tissue. These results underscore the importance of attributing biochemical properties to specific cell types in a tissue, a situation not paralleled in the interpretation of data from in vitro models.

UR - http://www.scopus.com/inward/record.url?scp=0031454189&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031454189&partnerID=8YFLogxK

M3 - Article

C2 - 9407974

AN - SCOPUS:0031454189

VL - 57

SP - 5605

EP - 5609

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 24

ER -

Varon D, Jiang C, Hedican C, Dome JS, Umbricht CB, Carey LA et al. Telomerase activity in the normal and neoplastic rat mammary gland. Cancer Research. 1997 Dec 15;57(24):5605-5609.