Telomere shortening and decreased replicative potential, contrasted by continued proliferation of telomerase-positive CD8+CD2810 T cells in patients with systemic lupus erythematosus

Motoko Honda, Emebet Mengesha, Shirley Albano-Aluquin, W. Stephen Nichols, Daniel J. Wallace, Alan Metzger, James R. Klinenberg, Mariana Linker-Israeli

Research output: Contribution to journalArticle

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Abstract

To evaluate whether the immune system of systemic lupus erythematosus (SLE) patients shows features of premature aging, we compared telomere length and proliferative potential of SLE peripheral blood mononuclear cells (PBMC) (N = 90) to those of controls (N = 64). SLE samples showed accelerated loss of telomeric DNA (P = 0.00008) and higher levels of senescent (≤5 kb) telomeric DNA (P = 0.00003). Viability cell counts and CFSE tracking in 6-week-old cell cultures indicated that SLE PBMC (CD8+ and CD4+ T cells) underwent fewer mitotic cycles and had shorter telomeres than controls (P = 0.04). However, a CD8+CD2810 T cell subset expanded preferentially in SLE-derived bulk cultures (P = 0.0009), preserved telomeric DNA (P = 0.01 vs entire CD8+), and displayed telomerase activity [2.1 telomerase arbitrary units (TAU) vs 0.5 TAU in CD8+CD28hi cells and 0.3 TAU in bulk PBMC; P = 0.05]. These T cell anomalies could be due to chronic in vivo stimulation of the immune system and may contribute to the immune dysregulation found in SLE.

Original languageEnglish (US)
Pages (from-to)211-221
Number of pages11
JournalClinical Immunology and Immunopathology
Volume99
Issue number2
StatePublished - Dec 1 2001

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Telomere Shortening
Telomerase
Systemic Lupus Erythematosus
T-Lymphocytes
Blood Cells
Telomere
Immune System
DNA
Premature Aging
T-Lymphocyte Subsets
Cell Culture Techniques
Cell Count

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

Cite this

Honda, Motoko ; Mengesha, Emebet ; Albano-Aluquin, Shirley ; Stephen Nichols, W. ; Wallace, Daniel J. ; Metzger, Alan ; Klinenberg, James R. ; Linker-Israeli, Mariana. / Telomere shortening and decreased replicative potential, contrasted by continued proliferation of telomerase-positive CD8+CD2810 T cells in patients with systemic lupus erythematosus. In: Clinical Immunology and Immunopathology. 2001 ; Vol. 99, No. 2. pp. 211-221.
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abstract = "To evaluate whether the immune system of systemic lupus erythematosus (SLE) patients shows features of premature aging, we compared telomere length and proliferative potential of SLE peripheral blood mononuclear cells (PBMC) (N = 90) to those of controls (N = 64). SLE samples showed accelerated loss of telomeric DNA (P = 0.00008) and higher levels of senescent (≤5 kb) telomeric DNA (P = 0.00003). Viability cell counts and CFSE tracking in 6-week-old cell cultures indicated that SLE PBMC (CD8+ and CD4+ T cells) underwent fewer mitotic cycles and had shorter telomeres than controls (P = 0.04). However, a CD8+CD2810 T cell subset expanded preferentially in SLE-derived bulk cultures (P = 0.0009), preserved telomeric DNA (P = 0.01 vs entire CD8+), and displayed telomerase activity [2.1 telomerase arbitrary units (TAU) vs 0.5 TAU in CD8+CD28hi cells and 0.3 TAU in bulk PBMC; P = 0.05]. These T cell anomalies could be due to chronic in vivo stimulation of the immune system and may contribute to the immune dysregulation found in SLE.",
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Telomere shortening and decreased replicative potential, contrasted by continued proliferation of telomerase-positive CD8+CD2810 T cells in patients with systemic lupus erythematosus. / Honda, Motoko; Mengesha, Emebet; Albano-Aluquin, Shirley; Stephen Nichols, W.; Wallace, Daniel J.; Metzger, Alan; Klinenberg, James R.; Linker-Israeli, Mariana.

In: Clinical Immunology and Immunopathology, Vol. 99, No. 2, 01.12.2001, p. 211-221.

Research output: Contribution to journalArticle

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