Template fabrication of proten-functionalized gold-polypyrrole-gold segmented nanowires

Rose M. Hernández, Lee Richter, Steve Semancik, Stephan Stranick, Thomas E. Mallouk

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Gold-capped, protein-modified polypyrrole (Ppy) nanowires were grown electrochemically using porous aluminum oxide as a template. The effects of the conditions of electrochemical synthesis on Ppy growth and protein (avidin or streptavidin) incorporation were studied. Streptavidin-modified nanowires grown at constant potential had better electrochemical properties and equilibrated faster when exposed to fluorescently labeled biotin than did nanowires grown by potential cycling. Solution pH had little effect on protein incorporation; however, higher pH provided slower but more reproducible growth rate of the Ppy segments. The best conditions for synthesis of streptavidin-modified Ppy nanowires were constant potential deposition at 0.75 V vs SCE in a phosphate buffer saline solution at pH 9. This method provides a straightforward route to nanowires of controlled length that can incorporate proteins for use in nanowire-based biosensors or in nanoparticle assembly through biomolecular interactions.

Original languageEnglish (US)
Pages (from-to)3431-3438
Number of pages8
JournalChemistry of Materials
Volume16
Issue number18
DOIs
StatePublished - Sep 7 2004

Fingerprint

Polypyrroles
Gold
Nanowires
Proteins
Fabrication
Streptavidin
Avidin
Aluminum Oxide
Biotin
polypyrrole
Electrochemical properties
Biosensors
Sodium Chloride
Buffers
Phosphates
Nanoparticles
Aluminum
Oxides

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

Cite this

Hernández, R. M., Richter, L., Semancik, S., Stranick, S., & Mallouk, T. E. (2004). Template fabrication of proten-functionalized gold-polypyrrole-gold segmented nanowires. Chemistry of Materials, 16(18), 3431-3438. https://doi.org/10.1021/cm0496265
Hernández, Rose M. ; Richter, Lee ; Semancik, Steve ; Stranick, Stephan ; Mallouk, Thomas E. / Template fabrication of proten-functionalized gold-polypyrrole-gold segmented nanowires. In: Chemistry of Materials. 2004 ; Vol. 16, No. 18. pp. 3431-3438.
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Hernández, RM, Richter, L, Semancik, S, Stranick, S & Mallouk, TE 2004, 'Template fabrication of proten-functionalized gold-polypyrrole-gold segmented nanowires', Chemistry of Materials, vol. 16, no. 18, pp. 3431-3438. https://doi.org/10.1021/cm0496265

Template fabrication of proten-functionalized gold-polypyrrole-gold segmented nanowires. / Hernández, Rose M.; Richter, Lee; Semancik, Steve; Stranick, Stephan; Mallouk, Thomas E.

In: Chemistry of Materials, Vol. 16, No. 18, 07.09.2004, p. 3431-3438.

Research output: Contribution to journalArticle

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Hernández RM, Richter L, Semancik S, Stranick S, Mallouk TE. Template fabrication of proten-functionalized gold-polypyrrole-gold segmented nanowires. Chemistry of Materials. 2004 Sep 7;16(18):3431-3438. https://doi.org/10.1021/cm0496265