Temporal gene regulation of the channel catfish virus (Ictalurid herpesvirus 1)

Suming Huang, Larry A. Hanson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To identify promoter regions that impart differential temporal regulation of channel catfish virus (CCV) genes, the transcriptional kinetics of an immediate-early gene and prospective early and late genes were characterized. A cDNA clone, designated IE3C, representing a third immediate- early transcript was identified. The 5' end of the IE3C transcript was mapped to nucleotides 15,368 and 131,043 in the terminal repeat regions of the CCV genome. The full length of the transcript represented by the IE3C clone is 1,412 bp, and it most likely codes for the protein specified by open reading frame (ORF) 12. The putative product of ORF12 contains a consensus RING finger metal binding motif (C3HC4 structure). Temporal expression studies, in conjunction with protein synthesis and DNA replication inhibition, demonstrated that the IE3C transcript belongs to an immediate-early kinetic class, the ORF5 transcript is a member of the early kinetic class, and ORF39 and ORF46 are true late-kinetic-class genes. Additionally, we demonstrated that ORF38 transcription overlaps ORF39 and the products presumably share the same poly(A) signal. The 5' ends of the transcripts encoding ORF38, ORF39, 'and ORF46 were mapped to nucleotides 44,862, 45,254, and 59,644, respectively, and potential transcriptional control elements were located.

Original languageEnglish (US)
Pages (from-to)1910-1917
Number of pages8
JournalJournal of virology
Volume72
Issue number3
StatePublished - Mar 1 1998

Fingerprint

Ictalurivirus
Ictalurid herpesvirus 1
kinetics
Genes
genes
Nucleotides
Clone Cells
nucleotides
clones
Poly A
Immediate-Early Genes
terminal repeat sequences
Terminal Repeat Sequences
DNA replication
DNA Replication
Genetic Promoter Regions
Open Reading Frames
open reading frames
Proteins
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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abstract = "To identify promoter regions that impart differential temporal regulation of channel catfish virus (CCV) genes, the transcriptional kinetics of an immediate-early gene and prospective early and late genes were characterized. A cDNA clone, designated IE3C, representing a third immediate- early transcript was identified. The 5' end of the IE3C transcript was mapped to nucleotides 15,368 and 131,043 in the terminal repeat regions of the CCV genome. The full length of the transcript represented by the IE3C clone is 1,412 bp, and it most likely codes for the protein specified by open reading frame (ORF) 12. The putative product of ORF12 contains a consensus RING finger metal binding motif (C3HC4 structure). Temporal expression studies, in conjunction with protein synthesis and DNA replication inhibition, demonstrated that the IE3C transcript belongs to an immediate-early kinetic class, the ORF5 transcript is a member of the early kinetic class, and ORF39 and ORF46 are true late-kinetic-class genes. Additionally, we demonstrated that ORF38 transcription overlaps ORF39 and the products presumably share the same poly(A) signal. The 5' ends of the transcripts encoding ORF38, ORF39, 'and ORF46 were mapped to nucleotides 44,862, 45,254, and 59,644, respectively, and potential transcriptional control elements were located.",
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Temporal gene regulation of the channel catfish virus (Ictalurid herpesvirus 1). / Huang, Suming; Hanson, Larry A.

In: Journal of virology, Vol. 72, No. 3, 01.03.1998, p. 1910-1917.

Research output: Contribution to journalArticle

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