Tetrandrine inhibits signal-induced NF-κB activation in rat alveolar macrophages

Fei Chen, Shaocong Sun, Douglas C. Kuhn, Yongju Lu, Lesley J. Gaydos, Xianglin Shi, Laurence M. Demers

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Tetrandrine is a bisbenzylisoquinoline alkaloid isolated from a natural Chinese herbal medicine. While this alkaloid has been shown to exhibit antifibrotic and anti-inflammatory activities, its mechanism of action is unknown. The present study was designed to investigate the inhibitory effect of tetrandrine on NF-κB activation in the alveolar macrophage. Three different provocative stimuli were used to activate NF-κB in these cells. The results indicate that tetrandrine can inhibit the activation of NF-κB and NF-κB-dependent reporter gene expression by LPS, PMA, and silica in a dose-dependent manner. In contrast, at the doses used, tetrandrine did not interfere with Sp-1 DNA binding activity or Sp-1-dependent reporter gene expression in these cells. Western blot analysis suggests that the inhibitory effect of tetrandrine on NF-κB activation can be attributed to its ability to suppress signal-induced degradation 1κBα, a cytoplasmic inhibitor of the NF-κB transcription factor.

Original languageEnglish (US)
Pages (from-to)99-102
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume231
Issue number1
DOIs
StatePublished - Feb 3 1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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