TGF-α and IGF-I effects on calcium ion transients in human breast cancer cells

Ransome Etindi, Andrea Manni

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In order to evaluate the potential role of calcium as an intracellular messenger for IGF-I and TGF-α action on breast cancer cell proliferation, we determined whether these growth factors induce any change in [Ca2+]i using fura-2 loaded cells. The hormone independent BT-20 and MDA-MB-231 cells were refractory to the mitogenic actions of exogenously added IGF-I and TGF-α. TGF-α administration, however, stimulated [Ca2+]i transients in the BT-20 cells. IGF-I and TGF-α stimulated DNA synthesis in the MCF-7 and T47D cells. These growth factors did not, however, stimulate any changes in [Ca2+]i in these cells. These data support the idea that receptor-mediated phospholipid hydrolysis does not serve a major signalling function for driving human breast cancer cells into DNA synthesis.

Original languageEnglish (US)
Pages (from-to)131-137
Number of pages7
JournalCancer Letters
Volume66
Issue number2
DOIs
StatePublished - Sep 30 1992

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Insulin-Like Growth Factor I
Ions
Breast Neoplasms
Calcium
Intercellular Signaling Peptides and Proteins
Fura-2
DNA
MCF-7 Cells
Phospholipids
Hydrolysis
Cell Proliferation
Hormones

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

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abstract = "In order to evaluate the potential role of calcium as an intracellular messenger for IGF-I and TGF-α action on breast cancer cell proliferation, we determined whether these growth factors induce any change in [Ca2+]i using fura-2 loaded cells. The hormone independent BT-20 and MDA-MB-231 cells were refractory to the mitogenic actions of exogenously added IGF-I and TGF-α. TGF-α administration, however, stimulated [Ca2+]i transients in the BT-20 cells. IGF-I and TGF-α stimulated DNA synthesis in the MCF-7 and T47D cells. These growth factors did not, however, stimulate any changes in [Ca2+]i in these cells. These data support the idea that receptor-mediated phospholipid hydrolysis does not serve a major signalling function for driving human breast cancer cells into DNA synthesis.",
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TGF-α and IGF-I effects on calcium ion transients in human breast cancer cells. / Etindi, Ransome; Manni, Andrea.

In: Cancer Letters, Vol. 66, No. 2, 30.09.1992, p. 131-137.

Research output: Contribution to journalArticle

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