The α9β1 integrin enhances cell migration by polyamine-mediated modulation of an inward-rectifier potassium channel

Gregory W. DeHart, Taihao Jin, Diane E. McCloskey, Anthony E. Pegg, Dean Sheppard

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The α9β1 integrin accelerates cell migration through binding of spermidine/spermine acetyltransferase (SSAT) to the α9 cytoplasmic domain. We now show that SSAT enhances α9-mediated migration specifically through catabolism of spermidine and/or spermine. Because spermine and spermidine are effective blockers of K+ ion efflux through inward-rectifier K + (Kir) channels, we examined the involvement of Kir channels in this pathway. The Kir channel inhibitor, barium, or knockdown of a single subunit, Kir4.2, specifically inhibited α9-dependent cell migration. α9β1 and Kir4.2 colocalized in focal adhesions at the leading edge of migrating cells and inhibition or knockdown of Kir4.2 caused reduced persistence and an increased number of lamellipodial extensions in cells migrating on an α9β1 ligand. These results identify a pathway through which the α9 integrin subunit stimulates cell migration by localized polyamine catabolism and modulation of Kir channel function.

Original languageEnglish (US)
Pages (from-to)7188-7193
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number20
DOIs
StatePublished - May 20 2008

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Inwardly Rectifying Potassium Channel
Spermine
Polyamines
Integrins
Cell Movement
Spermidine
Focal Adhesions
Barium
Ions
Ligands
diamine N-acetyltransferase

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "The α9β1 integrin accelerates cell migration through binding of spermidine/spermine acetyltransferase (SSAT) to the α9 cytoplasmic domain. We now show that SSAT enhances α9-mediated migration specifically through catabolism of spermidine and/or spermine. Because spermine and spermidine are effective blockers of K+ ion efflux through inward-rectifier K + (Kir) channels, we examined the involvement of Kir channels in this pathway. The Kir channel inhibitor, barium, or knockdown of a single subunit, Kir4.2, specifically inhibited α9-dependent cell migration. α9β1 and Kir4.2 colocalized in focal adhesions at the leading edge of migrating cells and inhibition or knockdown of Kir4.2 caused reduced persistence and an increased number of lamellipodial extensions in cells migrating on an α9β1 ligand. These results identify a pathway through which the α9 integrin subunit stimulates cell migration by localized polyamine catabolism and modulation of Kir channel function.",
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The α9β1 integrin enhances cell migration by polyamine-mediated modulation of an inward-rectifier potassium channel. / DeHart, Gregory W.; Jin, Taihao; McCloskey, Diane E.; Pegg, Anthony E.; Sheppard, Dean.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 20, 20.05.2008, p. 7188-7193.

Research output: Contribution to journalArticle

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