The ADP-ribosyltransferase PARP10/ARTD10 interacts with proliferating cell nuclear antigen (PCNA) and is required for DNA damage tolerance

Claudia M. Nicolae, Erin R. Aho, Alexander H.S. Vlahos, Katherine N. Choe, Subhajyoti De, Georgios I. Karras, George Lucian Moldovan

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

All cells rely on genomic stability mechanisms to protect against DNA alterations. PCNA is a master regulator of DNA replication and S-phase-coupled repair. PCNA post-translational modifications by ubiquitination and SUMOylation dictate how cells stabilize and re-start replication forks stalled at sites of damaged DNA.PCNAmono-ubiquitination recruits low fidelity DNA polymerases to promote error-prone replication across DNA lesions. Here, we identify the mono-ADP-ribosyltransferase PARP10/ARTD10 as a novelPCNAbinding partner. PARP10 knockdown results in genomic instability and DNA damage hypersensitivity. Importantly, we show that PARP10 binding toPCNAis required for translesionDNAsynthesis. Our work identifies a novel PCNA-linked mechanism for genome protection, centered on post-translational modification by mono-ADP-ribosylation.

Original languageEnglish (US)
Pages (from-to)13627-13637
Number of pages11
JournalJournal of Biological Chemistry
Volume289
Issue number19
DOIs
StatePublished - Jan 1 2014

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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