We present the changes to our published serum TCDD method including: 1) lower amount of serum required (100 g) for normal concentration levels to be measured; 2) addition of an external standard which allows for each analysis the calculation of internal standard recovery, the isomer specificity, and 10,000 RP at the time of peak detection; 3) more stringent QC requirements for a valid TCDD result; and 4) additional measures to protect against carryover from sample-to-sample. We also present a method validation and performance evaluation, along with QC results and ranges of concentration values that we determined in over 1,000 serum samples from Agent Orange, Ranch Hand, and NIOSH Worker Studies.
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Environmental Chemistry
- Health, Toxicology and Mutagenesis