The analytical methodology and method performance for the determination of 2,3,7,8-TCDD in serum for the Vietnam Veteran Agent Orange validation study, the ranch hand validation and half-life studies, and selected Niosh worker studies

Donald G. Patterson, Wayman E. Turner, Louis R. Alexander, Sandra Isaacs, Larry L. Needham

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We present the changes to our published serum TCDD method including: 1) lower amount of serum required (100 g) for normal concentration levels to be measured; 2) addition of an external standard which allows for each analysis the calculation of internal standard recovery, the isomer specificity, and 10,000 RP at the time of peak detection; 3) more stringent QC requirements for a valid TCDD result; and 4) additional measures to protect against carryover from sample-to-sample. We also present a method validation and performance evaluation, along with QC results and ranges of concentration values that we determined in over 1,000 serum samples from Agent Orange, Ranch Hand, and NIOSH Worker Studies.

Original languageEnglish (US)
Pages (from-to)875-882
Number of pages8
JournalChemosphere
Volume18
Issue number1-6
DOIs
StatePublished - 1989

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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