The arabidopsis cellulose synthase complex: A proposed hexamer of cesa trimers in an equimolar stoichiometryw

Joseph L. Hill, Mustafa B. Hammudi, Ming Tien

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Cellulose is the most abundant renewable polymer on Earth and a major component of the plant cell wall. In vascular plants, cellulose synthesis is catalyzed by a large, plasma membrane-localized cellulose synthase complex (CSC), visualized as a hexameric rosette structure. Three unique cellulose synthase (CESA) isoforms are required for CSC assembly and function. However, elucidation of either the number or stoichiometry of CESAs within the CSC has remained elusive. In this study, we show a 1:1:1 stoichiometry between the three Arabidopsis thaliana secondary cell wall isozymes: CESA4, CESA7, and CESA8. This ratio was determined utilizing a simple but elegant method of quantitative immunoblotting using isoform-specific antibodies and 35S-labeled protein standards for each CESA. Additionally, the observed equimolar stoichiometry was found to be fixed along the axis of the stem, which represents a developmental gradient. Our results complement recent spectroscopic analyses pointing toward an 18-chain cellulose microfibril. Taken together, we propose that the CSC is composed of a hexamer of catalytically active CESA trimers, with each CESA in equimolar amounts. This finding is a crucial advance in understanding how CESAs integrate to form higher order complexes, which is a key determinate of cellulose microfibril and cell wall properties

Original languageEnglish (US)
Pages (from-to)4834-4842
Number of pages9
JournalPlant Cell
Volume26
Issue number12
DOIs
StatePublished - Jan 1 2014

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cellulose synthase
Arabidopsis
Cellulose
cellulose
stoichiometry
Cell Wall
Microfibrils
cell walls
Protein Isoforms
Plant Cells
immunoblotting
Immunoblotting
vascular plants
Isoenzymes
Blood Vessels
quantitative analysis
isozymes
polymers
Polymers
complement

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

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abstract = "Cellulose is the most abundant renewable polymer on Earth and a major component of the plant cell wall. In vascular plants, cellulose synthesis is catalyzed by a large, plasma membrane-localized cellulose synthase complex (CSC), visualized as a hexameric rosette structure. Three unique cellulose synthase (CESA) isoforms are required for CSC assembly and function. However, elucidation of either the number or stoichiometry of CESAs within the CSC has remained elusive. In this study, we show a 1:1:1 stoichiometry between the three Arabidopsis thaliana secondary cell wall isozymes: CESA4, CESA7, and CESA8. This ratio was determined utilizing a simple but elegant method of quantitative immunoblotting using isoform-specific antibodies and 35S-labeled protein standards for each CESA. Additionally, the observed equimolar stoichiometry was found to be fixed along the axis of the stem, which represents a developmental gradient. Our results complement recent spectroscopic analyses pointing toward an 18-chain cellulose microfibril. Taken together, we propose that the CSC is composed of a hexamer of catalytically active CESA trimers, with each CESA in equimolar amounts. This finding is a crucial advance in understanding how CESAs integrate to form higher order complexes, which is a key determinate of cellulose microfibril and cell wall properties",
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The arabidopsis cellulose synthase complex : A proposed hexamer of cesa trimers in an equimolar stoichiometryw. / Hill, Joseph L.; Hammudi, Mustafa B.; Tien, Ming.

In: Plant Cell, Vol. 26, No. 12, 01.01.2014, p. 4834-4842.

Research output: Contribution to journalArticle

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