The archetype γ-class carbonic anhydrase (cam) contains iron when synthesized in vivo

Sheridan R. MacAuley, Sabrina A. Zimmerman, Ethel E. Apolinario, Caryn Evilia, Ya Ming Hou, James Gregory Ferry, Kevin R. Sowers

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A recombinant protein overproduction system was developed in Methanosarcina acetiVorans to facilitate biochemical characterization of oxygen-sensitive metal-loenzymes from strictly anaerobic species in the Archaea domain. The system was used to overproduce the archetype of the independently evolved γ-class carbonic anhydrase. The overproduced enzyme was oxygen sensitive and had full incorporation of iron instead of zinc observed when overproduced in Escherichia coli. This, the first report of in vivo iron incorporation for any carbonic anhydrase, supports the need to reevaluate the role of iron in all classes of carbonic anhydrases derived from anaerobic environments.

Original languageEnglish (US)
Pages (from-to)817-819
Number of pages3
JournalBiochemistry
Volume48
Issue number5
DOIs
StatePublished - Feb 10 2009

Fingerprint

Carbonic Anhydrases
Cams
Iron
Methanosarcina
Oxygen
Archaea
Recombinant Proteins
Escherichia coli
Zinc
Metals
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

MacAuley, S. R., Zimmerman, S. A., Apolinario, E. E., Evilia, C., Hou, Y. M., Ferry, J. G., & Sowers, K. R. (2009). The archetype γ-class carbonic anhydrase (cam) contains iron when synthesized in vivo. Biochemistry, 48(5), 817-819. https://doi.org/10.1021/bi802246s
MacAuley, Sheridan R. ; Zimmerman, Sabrina A. ; Apolinario, Ethel E. ; Evilia, Caryn ; Hou, Ya Ming ; Ferry, James Gregory ; Sowers, Kevin R. / The archetype γ-class carbonic anhydrase (cam) contains iron when synthesized in vivo. In: Biochemistry. 2009 ; Vol. 48, No. 5. pp. 817-819.
@article{105aaef101ec41bab65ee1a6e83ebef8,
title = "The archetype γ-class carbonic anhydrase (cam) contains iron when synthesized in vivo",
abstract = "A recombinant protein overproduction system was developed in Methanosarcina acetiVorans to facilitate biochemical characterization of oxygen-sensitive metal-loenzymes from strictly anaerobic species in the Archaea domain. The system was used to overproduce the archetype of the independently evolved γ-class carbonic anhydrase. The overproduced enzyme was oxygen sensitive and had full incorporation of iron instead of zinc observed when overproduced in Escherichia coli. This, the first report of in vivo iron incorporation for any carbonic anhydrase, supports the need to reevaluate the role of iron in all classes of carbonic anhydrases derived from anaerobic environments.",
author = "MacAuley, {Sheridan R.} and Zimmerman, {Sabrina A.} and Apolinario, {Ethel E.} and Caryn Evilia and Hou, {Ya Ming} and Ferry, {James Gregory} and Sowers, {Kevin R.}",
year = "2009",
month = "2",
day = "10",
doi = "10.1021/bi802246s",
language = "English (US)",
volume = "48",
pages = "817--819",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "5",

}

MacAuley, SR, Zimmerman, SA, Apolinario, EE, Evilia, C, Hou, YM, Ferry, JG & Sowers, KR 2009, 'The archetype γ-class carbonic anhydrase (cam) contains iron when synthesized in vivo', Biochemistry, vol. 48, no. 5, pp. 817-819. https://doi.org/10.1021/bi802246s

The archetype γ-class carbonic anhydrase (cam) contains iron when synthesized in vivo. / MacAuley, Sheridan R.; Zimmerman, Sabrina A.; Apolinario, Ethel E.; Evilia, Caryn; Hou, Ya Ming; Ferry, James Gregory; Sowers, Kevin R.

In: Biochemistry, Vol. 48, No. 5, 10.02.2009, p. 817-819.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The archetype γ-class carbonic anhydrase (cam) contains iron when synthesized in vivo

AU - MacAuley, Sheridan R.

AU - Zimmerman, Sabrina A.

AU - Apolinario, Ethel E.

AU - Evilia, Caryn

AU - Hou, Ya Ming

AU - Ferry, James Gregory

AU - Sowers, Kevin R.

PY - 2009/2/10

Y1 - 2009/2/10

N2 - A recombinant protein overproduction system was developed in Methanosarcina acetiVorans to facilitate biochemical characterization of oxygen-sensitive metal-loenzymes from strictly anaerobic species in the Archaea domain. The system was used to overproduce the archetype of the independently evolved γ-class carbonic anhydrase. The overproduced enzyme was oxygen sensitive and had full incorporation of iron instead of zinc observed when overproduced in Escherichia coli. This, the first report of in vivo iron incorporation for any carbonic anhydrase, supports the need to reevaluate the role of iron in all classes of carbonic anhydrases derived from anaerobic environments.

AB - A recombinant protein overproduction system was developed in Methanosarcina acetiVorans to facilitate biochemical characterization of oxygen-sensitive metal-loenzymes from strictly anaerobic species in the Archaea domain. The system was used to overproduce the archetype of the independently evolved γ-class carbonic anhydrase. The overproduced enzyme was oxygen sensitive and had full incorporation of iron instead of zinc observed when overproduced in Escherichia coli. This, the first report of in vivo iron incorporation for any carbonic anhydrase, supports the need to reevaluate the role of iron in all classes of carbonic anhydrases derived from anaerobic environments.

UR - http://www.scopus.com/inward/record.url?scp=61449255522&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61449255522&partnerID=8YFLogxK

U2 - 10.1021/bi802246s

DO - 10.1021/bi802246s

M3 - Article

C2 - 19187031

AN - SCOPUS:61449255522

VL - 48

SP - 817

EP - 819

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 5

ER -

MacAuley SR, Zimmerman SA, Apolinario EE, Evilia C, Hou YM, Ferry JG et al. The archetype γ-class carbonic anhydrase (cam) contains iron when synthesized in vivo. Biochemistry. 2009 Feb 10;48(5):817-819. https://doi.org/10.1021/bi802246s