TY - JOUR
T1 - The AWPM-19 family protein OsPM1 mediates abscisic acid influx and drought response in rice
AU - Yao, Lingya
AU - Cheng, Xuan
AU - Gu, Zongying
AU - Huang, Wei
AU - Li, Shou
AU - Wang, Linbo
AU - Wang, Yong Fei
AU - Xu, Ping
AU - Ma, Hong
AU - Ge, Xiaochun
N1 - Funding Information:
We thank Lizhong Xiong (Huazhong Agricultural University) for providing the 35Spro:OsbZIP46 transgenic seeds, Alexander M. Jones and Wolf B. Frommer (Carnegie Institution for Science) for providing the ABA FRET assay system, and Xinguang Zhu and Weining Sun (Shanghai Institute of Plant Physiology and Ecology) for providing the Li-6400XT photosynthesis system and anti-PIP2;7 antibody, respectively. We also thank Yaoqian Liu for vector construction and abiotic stress treatments. This research was supported by grants from the National Natural Science Foundation of China (31770274), from the Chinese Genetically Modified Organisms Major Project (2014ZX0800914B), and by the Chinese Ministry of Science and Technology 973 program (2012CB114300).
PY - 2018/6
Y1 - 2018/6
N2 - Abscisic acid (ABA) regulates plant stress responses and development. However, how the ABA signal is transmitted in response to stresses remains largely unclear, especially in monocots. In this study, we found that rice (Oryza sativa) OsPM1 (PLASMA MEMBRANE PROTEIN1), encoded by a gene of AWPM-19 like family, mediates ABA influx through the plasma membrane. OsPM1 is predominantly expressed in vascular tissues, guard cells, and mature embryos. Phenotypic analysis of overexpression, RNA interference (RNAi), and knockout (KO) lines showed that OsPM1 is involved in drought responses and seed germination regulation.3H-(±)ABA transport activity and fluorescence resonance energy transfer assays both demonstrated that OsPM1 facilitates ABA uptake into cells. The physiological isomer of ABA, (+)-ABA, is the preferred substrate of OsPM1. Higher ABA accumulation and faster stomatal closure in response to ABA treatment were observed in the overexpression lines compared with the wild-type control. Many ABA-responsive genes were upregulated more in the OsPM1-overexpression lines but less in the RNAi lines compared with wild-type plants. Further investigation revealed that OsPM1 expression is regulated by the AREB/ABF family transcription factor OsbZIP46. Our results thus revealed that OsPM1 is an ABA influx carrier that plays an important role in drought responses.
AB - Abscisic acid (ABA) regulates plant stress responses and development. However, how the ABA signal is transmitted in response to stresses remains largely unclear, especially in monocots. In this study, we found that rice (Oryza sativa) OsPM1 (PLASMA MEMBRANE PROTEIN1), encoded by a gene of AWPM-19 like family, mediates ABA influx through the plasma membrane. OsPM1 is predominantly expressed in vascular tissues, guard cells, and mature embryos. Phenotypic analysis of overexpression, RNA interference (RNAi), and knockout (KO) lines showed that OsPM1 is involved in drought responses and seed germination regulation.3H-(±)ABA transport activity and fluorescence resonance energy transfer assays both demonstrated that OsPM1 facilitates ABA uptake into cells. The physiological isomer of ABA, (+)-ABA, is the preferred substrate of OsPM1. Higher ABA accumulation and faster stomatal closure in response to ABA treatment were observed in the overexpression lines compared with the wild-type control. Many ABA-responsive genes were upregulated more in the OsPM1-overexpression lines but less in the RNAi lines compared with wild-type plants. Further investigation revealed that OsPM1 expression is regulated by the AREB/ABF family transcription factor OsbZIP46. Our results thus revealed that OsPM1 is an ABA influx carrier that plays an important role in drought responses.
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U2 - 10.1105/tpc.17.00770
DO - 10.1105/tpc.17.00770
M3 - Article
C2 - 29716991
AN - SCOPUS:85050096588
VL - 30
SP - 1258
EP - 1276
JO - Plant Cell
JF - Plant Cell
SN - 1040-4651
IS - 6
ER -