The BAX PCR Assay for Screening Listeria monocytogenes targets a partial putative gene lmo2234

Wei Zhang, Allison Hughes, Grier Wilt, Stephen John Knabel

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The BAX PCR for screening Listeria monocytogenes is a commercial PCR assay for specifically targeting L. monocytogenes, a foodborne pathogen that can contaminate a variety of foods and cause a potentially fatal disease, listeriosis, among high-risk populations. The high specificity (>98%) of this PCR assay is achieved by targeting a species-specific genomic region (∼400 bp) presumably found only in L. monocytogenes. In this study, the identity of the BAX PCR-targeted genomic region was determined by using PCR cloning, DNA sequencing, and basic local alignment search tool (BLAST) analysis of the amplicon sequences of an L. monocytogenes serotype 1/2a strain. BLAST analysis identified the BAX PCR amplicon (GenBank accession no. AY364605) as a 423-bp genomic region between nucleotides 224,409 and 224,831 in the genome of L. monocytogenes (serotype 1/2a strain EGD-e), including a 145-bp noncoding region and a 278-bp partial coding sequence of a putative gene, lmo2234. The translated amino acid sequence (92 amino acids) of this partial coding region is highly conserved between L. monocytogenes and Listeria innocua (93% homology). Reverse-position-specific BLAST analysis identified a conserved domain in Lmo2234 that was similar (95.3% aligned, E value = 9E-18) to the consensus amino acid sequence of sugar phosphate isomerases/epimerases (National Center for Biotechnology Information conserved domain database accession no. COG 1082.1, Io1E), indicating that Lmo2234 might be involved in bacterial carbohydrate transport and metabolism.

Original languageEnglish (US)
Pages (from-to)1507-1511
Number of pages5
JournalJournal of Food Protection
Volume67
Issue number7
DOIs
StatePublished - Jan 1 2004

Fingerprint

Listeria monocytogenes
screening
Polymerase Chain Reaction
assays
Genes
genes
genomics
Amino Acid Sequence
serotypes
amino acid sequences
sequence analysis
National Center for Biotechnology Information
Sugar Phosphates
sugar phosphates
Racemases and Epimerases
Information Centers
Listeriosis
Isomerases
Listeria
listeriosis

All Science Journal Classification (ASJC) codes

  • Food Science
  • Microbiology

Cite this

Zhang, Wei ; Hughes, Allison ; Wilt, Grier ; Knabel, Stephen John. / The BAX PCR Assay for Screening Listeria monocytogenes targets a partial putative gene lmo2234. In: Journal of Food Protection. 2004 ; Vol. 67, No. 7. pp. 1507-1511.
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abstract = "The BAX PCR for screening Listeria monocytogenes is a commercial PCR assay for specifically targeting L. monocytogenes, a foodborne pathogen that can contaminate a variety of foods and cause a potentially fatal disease, listeriosis, among high-risk populations. The high specificity (>98{\%}) of this PCR assay is achieved by targeting a species-specific genomic region (∼400 bp) presumably found only in L. monocytogenes. In this study, the identity of the BAX PCR-targeted genomic region was determined by using PCR cloning, DNA sequencing, and basic local alignment search tool (BLAST) analysis of the amplicon sequences of an L. monocytogenes serotype 1/2a strain. BLAST analysis identified the BAX PCR amplicon (GenBank accession no. AY364605) as a 423-bp genomic region between nucleotides 224,409 and 224,831 in the genome of L. monocytogenes (serotype 1/2a strain EGD-e), including a 145-bp noncoding region and a 278-bp partial coding sequence of a putative gene, lmo2234. The translated amino acid sequence (92 amino acids) of this partial coding region is highly conserved between L. monocytogenes and Listeria innocua (93{\%} homology). Reverse-position-specific BLAST analysis identified a conserved domain in Lmo2234 that was similar (95.3{\%} aligned, E value = 9E-18) to the consensus amino acid sequence of sugar phosphate isomerases/epimerases (National Center for Biotechnology Information conserved domain database accession no. COG 1082.1, Io1E), indicating that Lmo2234 might be involved in bacterial carbohydrate transport and metabolism.",
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The BAX PCR Assay for Screening Listeria monocytogenes targets a partial putative gene lmo2234. / Zhang, Wei; Hughes, Allison; Wilt, Grier; Knabel, Stephen John.

In: Journal of Food Protection, Vol. 67, No. 7, 01.01.2004, p. 1507-1511.

Research output: Contribution to journalArticle

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