The bioactive compounds α-chaconine and gallic acid in potato extracts decrease survival and induce apoptosis in LNCaP and PC3 prostate cancer cells

Lavanya Reddivari, Jairam Vanamala, Stephen H. Safe, J. Creighton Miller

Research output: Contribution to journalArticle

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Abstract

We recently reported that colored potato extracts and an anthocyanin rich fraction suppressed lymph-node carcinoma of the prostate (LNCaP) and prostate cancer-3 (PC-3) prostate cancer cell proliferation and induced apoptosis via caspase-dependent and caspase-independent pathways. Chlorogenic acid, caffeic acid, gallic acid, catechin, malvidin, and glycoalkaloids (α-chaconine and solanine) have now been identified as the major bioactive components of potato, and their effects on LNCaP and PC-3 cell proliferation and apoptosis have been investigated. α-chaconine (5 μg/ml) and gallic acid (15 μg/ml) exhibited potent antiproliferative properties and increased cyclin-dependent kinase inhibitor p27 levels in both cell lines. Both α-chaconine and gallic acid induced poly [adenosine diphosphate (ADP)] ribose polymerase cleavage and caspase-dependent apoptosis in LNCaP cells; however, caspase-independent apoptosis through nuclear translocation of endonuclease G was observed in both LNCaP and PC-3 cells. α-chaconine and gallic acid activated c-Jun N-terminal protein kinase (JNK), and this response played a major role in induction of caspase-dependent apoptosis in LNCaP cells; whereas modulation of JNK and mitogen-activated protein kinase did not affect α-chaconine- and gallic acid-induced caspase-independent apoptosis. These results suggest that apoptosis induced by whole potato extracts in prostate cancer cell lines may be in part due to α-chaconine and gallic acid.

Original languageEnglish (US)
Pages (from-to)601-610
Number of pages10
JournalNutrition and cancer
Volume62
Issue number5
DOIs
StatePublished - Jul 1 2010

Fingerprint

Gallic Acid
Solanum tuberosum
Caspases
Prostate
Prostatic Neoplasms
Lymph Nodes
Apoptosis
Carcinoma
JNK Mitogen-Activated Protein Kinases
Solanine
Cell Proliferation
Cyclin-Dependent Kinase Inhibitor p27
Poly Adenosine Diphosphate Ribose
Chlorogenic Acid
Cell Line
Anthocyanins
Catechin

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Reddivari, Lavanya ; Vanamala, Jairam ; Safe, Stephen H. ; Miller, J. Creighton. / The bioactive compounds α-chaconine and gallic acid in potato extracts decrease survival and induce apoptosis in LNCaP and PC3 prostate cancer cells. In: Nutrition and cancer. 2010 ; Vol. 62, No. 5. pp. 601-610.
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The bioactive compounds α-chaconine and gallic acid in potato extracts decrease survival and induce apoptosis in LNCaP and PC3 prostate cancer cells. / Reddivari, Lavanya; Vanamala, Jairam; Safe, Stephen H.; Miller, J. Creighton.

In: Nutrition and cancer, Vol. 62, No. 5, 01.07.2010, p. 601-610.

Research output: Contribution to journalArticle

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AU - Reddivari, Lavanya

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