The biological function of a fragment of the neurotrophic factor from pigment epithelium: Structural and functional homology with the differentiation factor of the HL-60 cell line

I. A. Kostanyan, S. S. Zhokhov, M. V. Astapova, S. M. Dranitsyna, A. P. Bogachuk, L. K. Baidakova, I. L. Rodionov, I. I. Baskin, O. N. Golubeva, J. Tombran-Tink, V. M. Lipkin

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

It was shown that the full-size neurotrophic factor from pigment epithelium (PEDF) induces the cell differentiation of the human promyelocyte leukemia cell line HL-60. A structural analysis of PEDF revealed in its C-terminal region a six-membered peptide fragment PEDF-(352-357) (PEDF-6) whose sequence is highly homologous to the 41-46 fragment of the active site of the human leukocyte differentiation factor HLDF (HLDF-6). The biological effect of PEDF and synthetic peptides PEDF-6 and HLDF-6 on the HL-60 cells and the early gastrula ectoderm of Xenopits laevis embryos was studied. On the basis of the structural and functional homologies of HLDF, PEDF, and their homologous peptides and the computer models of the spatial structures of the full-size PEDF and the PEDF with the C-terminal fragment split off tby the cleavage of the Leu380-Thr381 bond in the serpin loop, a hypothesis on the functional role of the serpin loop in PEDF was put forward.

Original languageEnglish (US)
Pages (from-to)505-511
Number of pages7
JournalRussian Journal of Bioorganic Chemistry
Volume26
Issue number9
StatePublished - Aug 2000

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'The biological function of a fragment of the neurotrophic factor from pigment epithelium: Structural and functional homology with the differentiation factor of the HL-60 cell line'. Together they form a unique fingerprint.

  • Cite this

    Kostanyan, I. A., Zhokhov, S. S., Astapova, M. V., Dranitsyna, S. M., Bogachuk, A. P., Baidakova, L. K., Rodionov, I. L., Baskin, I. I., Golubeva, O. N., Tombran-Tink, J., & Lipkin, V. M. (2000). The biological function of a fragment of the neurotrophic factor from pigment epithelium: Structural and functional homology with the differentiation factor of the HL-60 cell line. Russian Journal of Bioorganic Chemistry, 26(9), 505-511.