The BvgAS virulence control system regulates type III secretion in Bordetella bronchiseptica

Ming Huam Yuk, Eric Thomas Harvill, Jeff F. Miller

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

The BvgAS signal transduction system in Bordetella spp. mediates a transition between infectious (Bvg+) and non-infectious (Bvg-) phases by sensing environmental conditions and regulating gene expression. Using differential display, arbitrary-primed polymerase chain reaction (PCR), we identified a gene expressed in the Bvg+ phase of Bordetella bronchiseptica that shows a high degree of sequence similarity to a locus involved in providing energy for type III secretion in pathogenic Gram-negative bacteria (yscN in Yersinia spp.). We determined that the expression of this homologue in B. bronchiseptica (designated bscN) is regulated by bvg. Several open reading frames surrounding the bscN locus also show sequence similarity to loci encoding type III secretion apparatus components in other bacteria. An in-frame deletion of bscN in B. bronchiseptica leads to decreased secretion of several proteins, decreased cytotoxicity towards cultured cell lines and a defect in causing tyrosine dephosphorylation of specific proteins in infected cells in vitro. The deletion strain also revealed that bscN-mediated secretion is required for persistent colonization of the trachea in a rat infection model. Loci encoding type III secretion homologues were identified in four strains of B. pertussis and two strains of B. parapertussis. B. pertussis strain 18323 and an ovine isolate of B. parapertussis show significant transcription of the genes in vitro.

Original languageEnglish (US)
Pages (from-to)945-959
Number of pages15
JournalMolecular Microbiology
Volume28
Issue number5
DOIs
StatePublished - Jun 17 1998

Fingerprint

Bordetella bronchiseptica
Virulence
Whooping Cough
Bordetella
Yersinia
Trachea
Gram-Negative Bacteria
Open Reading Frames
Genes
Tyrosine
Cultured Cells
Signal Transduction
Sheep
Proteins
Bacteria
Gene Expression
Cell Line
Polymerase Chain Reaction
Infection
Type III Secretion Systems

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Yuk, Ming Huam ; Harvill, Eric Thomas ; Miller, Jeff F. / The BvgAS virulence control system regulates type III secretion in Bordetella bronchiseptica. In: Molecular Microbiology. 1998 ; Vol. 28, No. 5. pp. 945-959.
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The BvgAS virulence control system regulates type III secretion in Bordetella bronchiseptica. / Yuk, Ming Huam; Harvill, Eric Thomas; Miller, Jeff F.

In: Molecular Microbiology, Vol. 28, No. 5, 17.06.1998, p. 945-959.

Research output: Contribution to journalArticle

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