@article{0b53cd4499cc4b83b6469b55a1593b8b,
title = "The C-Terminal Domain of RNA Polymerase II Is a Multivalent Targeting Sequence that Supports Drosophila Development with Only Consensus Heptads",
abstract = "The C-terminal domain (CTD) of RNA polymerase II (Pol II) is composed of repeats of the consensus YSPTSPS and is an essential binding scaffold for transcription-associated factors. Metazoan CTDs have well-conserved lengths and sequence compositions arising from the evolution of divergent motifs, features thought to be essential for development. On the contrary, we show that a truncated CTD composed solely of YSPTSPS repeats supports Drosophila viability but that a CTD with enough YSPTSPS repeats to match the length of the wild-type Drosophila CTD is defective. Furthermore, a fluorescently tagged CTD lacking the rest of Pol II dynamically enters transcription compartments, indicating that the CTD functions as a signal sequence. However, CTDs with too many YSPTSPS repeats are more prone to localize to static nuclear foci separate from the chromosomes. We propose that the sequence complexity of the CTD offsets aberrant behavior caused by excessive repetitive sequences without compromising its targeting function.",
author = "Feiyue Lu and Bede Portz and Gilmour, {David S.}",
note = "Funding Information: We thank M. M. Rolls, C. M. Thomas, and Z. C. Lai for imaging support; C. Feng for CRISPR and live imaging suggestions; and D. G. Baumann, Y. Qiu, R. Dollinger, and J. C. Reese for comments on the manuscript. The Sgs3-GAL4, UAS-mCh-H2B/TM6 fly line was a generous gift from C. Peter Verrijzer. We thank the TRiP at Harvard Medical School ( NIH/NIGMS R01-GM084947 ) for providing the transgenic Rpb1 RNAi fly stock used in this study. This work was funded by NIH/NIGMS R01-GM047477 (to D.S.G.). Funding Information: We thank M. M. Rolls, C. M. Thomas, and Z. C. Lai for imaging support; C. Feng for CRISPR and live imaging suggestions; and D. G. Baumann, Y. Qiu, R. Dollinger, and J. C. Reese for comments on the manuscript. The Sgs3-GAL4, UAS-mCh-H2B/TM6 fly line was a generous gift from C. Peter Verrijzer. We thank the TRiP at Harvard Medical School (NIH/NIGMS R01-GM084947) for providing the transgenic Rpb1 RNAi fly stock used in this study. This work was funded by NIH/NIGMS R01-GM047477 (to D.S.G.). Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = mar,
day = "21",
doi = "10.1016/j.molcel.2019.01.008",
language = "English (US)",
volume = "73",
pages = "1232--1242.e4",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "6",
}