The C terminus of the polerovirus P5 readthrough domain limits virus infection to the phloem

Kari Anne Peter, Frederick Gildow, Peter Palukaitis, Stewart M. Gray

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Poleroviruses are restricted to vascular phloem tissues from which they are transmitted by their aphid vectors and are not transmissible mechanically. Phloem limitation has been attributed to the absence of virus proteins either facilitating movement or counteracting plant defense. The polerovirus capsid is composed of two forms of coat protein, the major P3 protein and the minor P3/P5 protein, a translational readthrough of P3. P3/P5 is required for insect transmission and acts in trans to facilitate long-distance virus movement in phloem tissue. Specific potato leafroll virus mutants lacking part or all of the P5 domain moved into and infected nonvascular mesophyll tissue when the source-sink relationship of the plant (Solanum sarrachoides) was altered by pruning, with the progeny virus now being transmissible mechanically. However, in a period of months, a phloem-specific distribution of the virus was reestablished in the absence of aphid transmission. Virus from the new phloem-limited infection showed compensatory mutations that would be expected to restore the production of full-length P3/P5 as well as the loss of mechanical transmissibility. The data support our hypothesis that phloem limitation in poleroviruses presumably does not result from a deficiency in the repertoire of virus genes but rather results from P3/P5 accumulation under selection in the infected plant, with the colateral effect of facilitating transmission by phloem-feeding aphid vectors.

Original languageEnglish (US)
Pages (from-to)5419-5429
Number of pages11
JournalJournal of virology
Volume83
Issue number11
DOIs
StatePublished - Jun 1 2009

Fingerprint

Luteoviridae
Polerovirus
Phloem
Virus Diseases
phloem
Viruses
viruses
Aphids
infection
Aphidoidea
Solanum sarrachoides
Potato leafroll virus
Solanum
source-sink relationships
Proteins
capsid
proteins
Capsid
Capsid Proteins
Solanum tuberosum

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Peter, Kari Anne ; Gildow, Frederick ; Palukaitis, Peter ; Gray, Stewart M. / The C terminus of the polerovirus P5 readthrough domain limits virus infection to the phloem. In: Journal of virology. 2009 ; Vol. 83, No. 11. pp. 5419-5429.
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The C terminus of the polerovirus P5 readthrough domain limits virus infection to the phloem. / Peter, Kari Anne; Gildow, Frederick; Palukaitis, Peter; Gray, Stewart M.

In: Journal of virology, Vol. 83, No. 11, 01.06.2009, p. 5419-5429.

Research output: Contribution to journalArticle

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