The Caenorhabditis elegans heterochronic gene lin-14 coordinates temporal progression and maturation in the egg-laying system

Ryan W. Johnson, Leah Y. Liu, Wendy Hanna-Rose, Helen M. Chamberlin

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Heterochronic genes function to ensure the timing of stage-specific developmental events in C. elegans. Mutations in these genes cause certain developmental programs to be executed in a precocious or retarded manner. Canonical precocious (loss-of-function) and retarded (gain-of-function) mutations in the lin-14 gene lead to elimination or reiteration of larval stage-specific cellular events. Here, we describe a hypomorphic, missense allele of lin-14, sa485. lin-14(sa485) hermaphrodites pass through normal larval stages, but exhibit asynchrony between vulval and gonadal maturation in the L4 larval stage. We show that a subtly precocious morphogenetic event in the vulva disrupts tissue synchrony and is followed by retarded vulval eversion. Additionally, uterine uv1 cell differentiation is retarded in lin-14(sa485) animals that exhibit delayed vulval eversion. Together, these experiments outline a function for LIN-14 in coordinating the temporal progression of development, which is separable from its role in regulating stage-specific events during C. elegans postembryonic development.

Original languageEnglish (US)
Pages (from-to)394-404
Number of pages11
JournalDevelopmental Dynamics
Volume238
Issue number2
DOIs
StatePublished - Feb 2009

All Science Journal Classification (ASJC) codes

  • Developmental Biology

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