The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice

Sung Cheol Koo, Man Soo Choi, Hyun Jin Chun, Dong Bum Shin, Bong Soo Park, Yul Ho Kim, Hyang Mi Park, Hak Soo Seo, Jong Tae Song, Kyu Young Kang, Dae Jin Yun, Woo Sik Chung, Moo Je Cho, Min Chul Kim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We previously isolated the OsCBT gene, which encodes a calmodulin (CaM)-binding protein, from a rice expression library constructed from fungal elicitor-treated rice suspension cells. In order to understand the function of OsCBT in rice, we isolated and characterized a T-DNA insertion mutant allele named oscbt-1. The oscbt-1 mutant exhibits reduced levels of OsCBT transcripts and no significant morphological changes compared to wild-type plant although the growth of the mutant is stunted. However, oscbt-1 mutants showed significant resistance to two major rice pathogens. The growth of the rice blast fungus Magnaporthe grisea, as well as the bacterial pathogen Xanthomonas oryzae pv. oryzae was significantly suppressed in oscbt-1 plants. Histochemical analysis indicated that the hypersensitive-response was induced in the oscbt-1 mutant in response to compatible strains of fungal pathogens. OsCBT expression was induced upon challenge with fungal elicitor. We also observed significant increase in the level of pathogenesis-related genes in the oscbt-1 mutant even under pathogen-free condition. Taken together, the results support an idea that OsCBT might act as a negative regulator on plant defense.

Original languageEnglish (US)
Pages (from-to)563-570
Number of pages8
JournalMolecules and cells
Volume27
Issue number5
DOIs
StatePublished - Jun 12 2009

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Calmodulin
Transcription Factors
Magnaporthe
Xanthomonas
Calmodulin-Binding Proteins
Growth
Oryza
Genes
Suspensions
Fungi
Alleles

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Koo, S. C., Choi, M. S., Chun, H. J., Shin, D. B., Park, B. S., Kim, Y. H., ... Kim, M. C. (2009). The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice. Molecules and cells, 27(5), 563-570. https://doi.org/10.1007/s10059-009-0081-4
Koo, Sung Cheol ; Choi, Man Soo ; Chun, Hyun Jin ; Shin, Dong Bum ; Park, Bong Soo ; Kim, Yul Ho ; Park, Hyang Mi ; Seo, Hak Soo ; Song, Jong Tae ; Kang, Kyu Young ; Yun, Dae Jin ; Chung, Woo Sik ; Cho, Moo Je ; Kim, Min Chul. / The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice. In: Molecules and cells. 2009 ; Vol. 27, No. 5. pp. 563-570.
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abstract = "We previously isolated the OsCBT gene, which encodes a calmodulin (CaM)-binding protein, from a rice expression library constructed from fungal elicitor-treated rice suspension cells. In order to understand the function of OsCBT in rice, we isolated and characterized a T-DNA insertion mutant allele named oscbt-1. The oscbt-1 mutant exhibits reduced levels of OsCBT transcripts and no significant morphological changes compared to wild-type plant although the growth of the mutant is stunted. However, oscbt-1 mutants showed significant resistance to two major rice pathogens. The growth of the rice blast fungus Magnaporthe grisea, as well as the bacterial pathogen Xanthomonas oryzae pv. oryzae was significantly suppressed in oscbt-1 plants. Histochemical analysis indicated that the hypersensitive-response was induced in the oscbt-1 mutant in response to compatible strains of fungal pathogens. OsCBT expression was induced upon challenge with fungal elicitor. We also observed significant increase in the level of pathogenesis-related genes in the oscbt-1 mutant even under pathogen-free condition. Taken together, the results support an idea that OsCBT might act as a negative regulator on plant defense.",
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Koo, SC, Choi, MS, Chun, HJ, Shin, DB, Park, BS, Kim, YH, Park, HM, Seo, HS, Song, JT, Kang, KY, Yun, DJ, Chung, WS, Cho, MJ & Kim, MC 2009, 'The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice', Molecules and cells, vol. 27, no. 5, pp. 563-570. https://doi.org/10.1007/s10059-009-0081-4

The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice. / Koo, Sung Cheol; Choi, Man Soo; Chun, Hyun Jin; Shin, Dong Bum; Park, Bong Soo; Kim, Yul Ho; Park, Hyang Mi; Seo, Hak Soo; Song, Jong Tae; Kang, Kyu Young; Yun, Dae Jin; Chung, Woo Sik; Cho, Moo Je; Kim, Min Chul.

In: Molecules and cells, Vol. 27, No. 5, 12.06.2009, p. 563-570.

Research output: Contribution to journalArticle

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T1 - The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice

AU - Koo, Sung Cheol

AU - Choi, Man Soo

AU - Chun, Hyun Jin

AU - Shin, Dong Bum

AU - Park, Bong Soo

AU - Kim, Yul Ho

AU - Park, Hyang Mi

AU - Seo, Hak Soo

AU - Song, Jong Tae

AU - Kang, Kyu Young

AU - Yun, Dae Jin

AU - Chung, Woo Sik

AU - Cho, Moo Je

AU - Kim, Min Chul

PY - 2009/6/12

Y1 - 2009/6/12

N2 - We previously isolated the OsCBT gene, which encodes a calmodulin (CaM)-binding protein, from a rice expression library constructed from fungal elicitor-treated rice suspension cells. In order to understand the function of OsCBT in rice, we isolated and characterized a T-DNA insertion mutant allele named oscbt-1. The oscbt-1 mutant exhibits reduced levels of OsCBT transcripts and no significant morphological changes compared to wild-type plant although the growth of the mutant is stunted. However, oscbt-1 mutants showed significant resistance to two major rice pathogens. The growth of the rice blast fungus Magnaporthe grisea, as well as the bacterial pathogen Xanthomonas oryzae pv. oryzae was significantly suppressed in oscbt-1 plants. Histochemical analysis indicated that the hypersensitive-response was induced in the oscbt-1 mutant in response to compatible strains of fungal pathogens. OsCBT expression was induced upon challenge with fungal elicitor. We also observed significant increase in the level of pathogenesis-related genes in the oscbt-1 mutant even under pathogen-free condition. Taken together, the results support an idea that OsCBT might act as a negative regulator on plant defense.

AB - We previously isolated the OsCBT gene, which encodes a calmodulin (CaM)-binding protein, from a rice expression library constructed from fungal elicitor-treated rice suspension cells. In order to understand the function of OsCBT in rice, we isolated and characterized a T-DNA insertion mutant allele named oscbt-1. The oscbt-1 mutant exhibits reduced levels of OsCBT transcripts and no significant morphological changes compared to wild-type plant although the growth of the mutant is stunted. However, oscbt-1 mutants showed significant resistance to two major rice pathogens. The growth of the rice blast fungus Magnaporthe grisea, as well as the bacterial pathogen Xanthomonas oryzae pv. oryzae was significantly suppressed in oscbt-1 plants. Histochemical analysis indicated that the hypersensitive-response was induced in the oscbt-1 mutant in response to compatible strains of fungal pathogens. OsCBT expression was induced upon challenge with fungal elicitor. We also observed significant increase in the level of pathogenesis-related genes in the oscbt-1 mutant even under pathogen-free condition. Taken together, the results support an idea that OsCBT might act as a negative regulator on plant defense.

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