The CDC25 Protein of Saccharomyces cerevisiae Promotes Exchange of Guanine Nucleotides Bound to Ras

Sara Jones, Marie Luce Vignais, James R. Broach

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132 Scopus citations

Abstract

The product of the CDC25 gene of Saccharomyces cerevisiae, in its capacity as an activator of the RAS/cyclic AMP pathway, is required for initiation of the cell cycle. In this report, we provide an identification of Cdc25p, the product of the CDC25 gene, and evidence that it promotes exchange of guanine nucleotides bound to Ras in vitro. Extracts of strains containing high levels of Cdc25p catalyze both removal of GDP from and the concurrent binding of GTP to Ras. This same activity is also obtained with an immunopurified Cdc25p-β-galactosidase fusion protein, suggesting that Cdc25p participates directly in the exchange reaction. This biochemical activity is consistent with previous genetic analysis of CDC25 function.

Original languageEnglish (US)
Pages (from-to)2641-2646
Number of pages6
JournalMolecular and cellular biology
Volume11
Issue number5
DOIs
StatePublished - May 1991

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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