The Cebpd (C/EBPδ) gene is induced by luteinizing hormones in ovarian theca and interstitial cells but is not essential for mouse ovary function

A. Mei Huang, Martina Rudelius, Shikha Sharan, Jan M. McAllister, Mark Raffeld, Lane K. Christenson, Esta Sterneck

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The CCAAT/enhancer binding protein (CEBP) family of transcription factors includes five genes. In the ovary, both Cebpa and Cebpb are essential for granulosa cell function. In this study we have explored the role of the Cebpd gene in ovarian physiology by expression and functional studies. Here we report that Cebpd (C/EBPδ) is expressed in the mouse ovary in a highly restricted temporal and spatial pattern. In response to luteinizing hormone (LH/hCG), CEBPD expression is transiently induced in interstitial cells and in theca cells of follicles from the primary to pre-ovulatory stage, and overlaps in part with expression of the alpha-smooth muscle actin protein. Efficient down-regulation of CEBPD was dependent on a functional Cebpb gene. Proliferating human theca cells in culture also express Cebpd. Cells from patients with polycystic ovarian syndrome (PCOS) exhibited higher Cebpd expression levels. However, deletion of Cebpd in mice had no overt effect on ovarian physiology and reproductive function. Very little is known at present about the molecular mechanisms underlying theca/ interstitial cell functions. The expression pattern of CEBPD reported here identifies a novel functional unit of mouse theca cells of primary through tertiary follicles responding to LH/hCG together with a subset of interstitial cells. This acute stimulation of CEBPD expression may be exploited to further characterize the hormonal regulation and function of theca and interstitial cells.

Original languageEnglish (US)
Article numbere1334
JournalPloS one
Volume2
Issue number12
DOIs
StatePublished - Dec 19 2007

Fingerprint

Theca Cells
luteinizing hormone
Luteinizing Hormone
Ovary
Genes
mice
Physiology
genes
cells
CCAAT-Enhancer-Binding Proteins
human chorionic gonadotropin
Cell culture
Muscle Proteins
Granulosa Cells
Polycystic Ovary Syndrome
physiology
Muscle
Actins
polycystic ovary syndrome
Transcription Factors

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Huang, A. Mei ; Rudelius, Martina ; Sharan, Shikha ; McAllister, Jan M. ; Raffeld, Mark ; Christenson, Lane K. ; Sterneck, Esta. / The Cebpd (C/EBPδ) gene is induced by luteinizing hormones in ovarian theca and interstitial cells but is not essential for mouse ovary function. In: PloS one. 2007 ; Vol. 2, No. 12.
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The Cebpd (C/EBPδ) gene is induced by luteinizing hormones in ovarian theca and interstitial cells but is not essential for mouse ovary function. / Huang, A. Mei; Rudelius, Martina; Sharan, Shikha; McAllister, Jan M.; Raffeld, Mark; Christenson, Lane K.; Sterneck, Esta.

In: PloS one, Vol. 2, No. 12, e1334, 19.12.2007.

Research output: Contribution to journalArticle

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