The central executioner of apoptosis

Multiple connections between protease activation and mitochondria in Fas/APO-1/CD95- and ceramide-induced apoptosis

Santos A. Susin, Naoufal Zamzami, Maria Castedo, Eric Daugas, Hong-Gang Wang, Stephan Geley, Florence Fassy, John C. Reed, Guido Kroemer

Research output: Contribution to journalArticle

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Abstract

According to current understanding, cytoplasmic events including activation of protease cascades and mitochondrial permeability transition (PT) participate in the control of nuclear apoptosis. However, the relationship between protease activation and PT has remained elusive. When apoptosis is induced by cross-linking of the Fas/APO-1/CD95 receptor, activation of interleukin-1β converting enzyme (ICE; caspase 1) or ICE-like enzymes precedes the disruption of the mitochondrial inner transmembrane potential (Δ Ψ(m)). In contrast, cytosolic CPP32/Yama/Apopain/caspase 3 activation, plasma membrane phosphatidyl serine exposure, and nuclear apoptosis only occur in cells in which the Δ Ψ(m) is fully disrupted. Transfection with the cowpox protease inhibitor crmA or culture in the presence of the synthetic ICE-specific inhibitor Ac-YVAD.cmk both prevent tbe Δ Ψ(m) collapse and subsequent apoptosis. Cytosols from anti-Fas-treated human lymphoma cells accumulate an activity that induces PT in isolated mitochondria in vitro and that is neutralized by crmA or Ac-YVAD.cmk. Recombimant purified ICE suffices to cause isolated mitochondria to undergo PT-like large amplitude swelling and to disrupt their Δ Ψ(m). In addition, ICE-treated mitochondria release an apoptosis-inducing factor (AIF) that induces apoptotic changes (chromatin condensation and oligonucleosomal DNA fragmentation) in isolated nuclei in vitro. AIF is a protease (or protease activator) that can be inhibited by the broad spectrum apoptosis inhibitor Z- VAD.fmk and that causes the proteolytical activation of CPP32. Although Bcl- 2 is a highly efficient inhibitor of mitochondrial alterations (large amplitude swelling + Δ Ψ(m) collapse + release of AIF) induced by prooxidants or cytosols from ceramide-treated cells, it has no effect on the ICE-induced mitochondrial PT and AIF release. These data connect a protease activation pathway with the mitochondrial phase of apoptosis regulation. In addition, they provide a plausible explanation of why Bcl-2 fails to interfere with Fas-triggered apoptosis in most cell types, yet prevents ceramide- and prooxidant-reduced apoptosis.

Original languageEnglish (US)
Pages (from-to)25-37
Number of pages13
JournalJournal of Experimental Medicine
Volume186
Issue number1
DOIs
StatePublished - Jul 7 1997

Fingerprint

Ceramides
Mitochondria
Peptide Hydrolases
Apoptosis Inducing Factor
Apoptosis
Permeability
Caspase 1
Caspase 3
Cytosol
Cowpox
Phosphatidylserines
DNA Fragmentation
Protease Inhibitors
Membrane Potentials
Chromatin
Transfection
Lymphoma
Cell Membrane
Enzymes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Susin, Santos A. ; Zamzami, Naoufal ; Castedo, Maria ; Daugas, Eric ; Wang, Hong-Gang ; Geley, Stephan ; Fassy, Florence ; Reed, John C. ; Kroemer, Guido. / The central executioner of apoptosis : Multiple connections between protease activation and mitochondria in Fas/APO-1/CD95- and ceramide-induced apoptosis. In: Journal of Experimental Medicine. 1997 ; Vol. 186, No. 1. pp. 25-37.
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The central executioner of apoptosis : Multiple connections between protease activation and mitochondria in Fas/APO-1/CD95- and ceramide-induced apoptosis. / Susin, Santos A.; Zamzami, Naoufal; Castedo, Maria; Daugas, Eric; Wang, Hong-Gang; Geley, Stephan; Fassy, Florence; Reed, John C.; Kroemer, Guido.

In: Journal of Experimental Medicine, Vol. 186, No. 1, 07.07.1997, p. 25-37.

Research output: Contribution to journalArticle

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T1 - The central executioner of apoptosis

T2 - Multiple connections between protease activation and mitochondria in Fas/APO-1/CD95- and ceramide-induced apoptosis

AU - Susin, Santos A.

AU - Zamzami, Naoufal

AU - Castedo, Maria

AU - Daugas, Eric

AU - Wang, Hong-Gang

AU - Geley, Stephan

AU - Fassy, Florence

AU - Reed, John C.

AU - Kroemer, Guido

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