The changing ratio of serum bioactive to immunoreactive follicle-stimulating hormone in normal men following treatment with a potent gonadotropin releasing hormone antagonist

Kristine D. Dah, Spyros N. Pavlou, William J. Kovacs, Aaron J.W. Hsueh

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70 Citations (Scopus)

Abstract

Using a recently developed granulosa cell aromatase bioassy (GAB), we measured serum bioactive follicle-stimulating hormone (bio-FSH) levels in 5 normal men after administration of a potent GnRH antagonist. Although only minimal suppression of immunoreactive FSH (immuno-FSH) was detected during administration of 20 mg of the antagonist, ([N-Ac-D-Nal(2)1D-pCl-Phe2 D-Trp3D-h(Arg(Et2)6 D-Ala10]GnRH, pronounced inhibition (79 to89%) of bio-FSH levels occurred. Concomitantly, the ratio of bio- to immuno-FSH levels dramatically decreased within 6 h after antagonist administration. These data reinforce earlier expectations that GnRH antagonists might be potential male contraceptives and provide the first report of changes in circulating bio- to immuno-FSH levels. The GAB assay will facilitate future studies on the biochemical mechanisms by which GnRH antagonists induce changes in the bioactivity of circulating FSH.

Original languageEnglish (US)
Pages (from-to)792-794
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Volume63
Issue number3
DOIs
StatePublished - Sep 1986

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Hormone Antagonists
Follicle Stimulating Hormone
Gonadotropin-Releasing Hormone
Aromatase
Granulosa Cells
Serum
Male Contraceptive Agents
Therapeutics
Bioactivity
Assays

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

@article{7828935a957449dea2317992d3f3a7e2,
title = "The changing ratio of serum bioactive to immunoreactive follicle-stimulating hormone in normal men following treatment with a potent gonadotropin releasing hormone antagonist",
abstract = "Using a recently developed granulosa cell aromatase bioassy (GAB), we measured serum bioactive follicle-stimulating hormone (bio-FSH) levels in 5 normal men after administration of a potent GnRH antagonist. Although only minimal suppression of immunoreactive FSH (immuno-FSH) was detected during administration of 20 mg of the antagonist, ([N-Ac-D-Nal(2)1D-pCl-Phe2 D-Trp3D-h(Arg(Et2)6 D-Ala10]GnRH, pronounced inhibition (79 to89{\%}) of bio-FSH levels occurred. Concomitantly, the ratio of bio- to immuno-FSH levels dramatically decreased within 6 h after antagonist administration. These data reinforce earlier expectations that GnRH antagonists might be potential male contraceptives and provide the first report of changes in circulating bio- to immuno-FSH levels. The GAB assay will facilitate future studies on the biochemical mechanisms by which GnRH antagonists induce changes in the bioactivity of circulating FSH.",
author = "Dah, {Kristine D.} and Pavlou, {Spyros N.} and Kovacs, {William J.} and Hsueh, {Aaron J.W.}",
year = "1986",
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volume = "63",
pages = "792--794",
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T1 - The changing ratio of serum bioactive to immunoreactive follicle-stimulating hormone in normal men following treatment with a potent gonadotropin releasing hormone antagonist

AU - Dah, Kristine D.

AU - Pavlou, Spyros N.

AU - Kovacs, William J.

AU - Hsueh, Aaron J.W.

PY - 1986/9

Y1 - 1986/9

N2 - Using a recently developed granulosa cell aromatase bioassy (GAB), we measured serum bioactive follicle-stimulating hormone (bio-FSH) levels in 5 normal men after administration of a potent GnRH antagonist. Although only minimal suppression of immunoreactive FSH (immuno-FSH) was detected during administration of 20 mg of the antagonist, ([N-Ac-D-Nal(2)1D-pCl-Phe2 D-Trp3D-h(Arg(Et2)6 D-Ala10]GnRH, pronounced inhibition (79 to89%) of bio-FSH levels occurred. Concomitantly, the ratio of bio- to immuno-FSH levels dramatically decreased within 6 h after antagonist administration. These data reinforce earlier expectations that GnRH antagonists might be potential male contraceptives and provide the first report of changes in circulating bio- to immuno-FSH levels. The GAB assay will facilitate future studies on the biochemical mechanisms by which GnRH antagonists induce changes in the bioactivity of circulating FSH.

AB - Using a recently developed granulosa cell aromatase bioassy (GAB), we measured serum bioactive follicle-stimulating hormone (bio-FSH) levels in 5 normal men after administration of a potent GnRH antagonist. Although only minimal suppression of immunoreactive FSH (immuno-FSH) was detected during administration of 20 mg of the antagonist, ([N-Ac-D-Nal(2)1D-pCl-Phe2 D-Trp3D-h(Arg(Et2)6 D-Ala10]GnRH, pronounced inhibition (79 to89%) of bio-FSH levels occurred. Concomitantly, the ratio of bio- to immuno-FSH levels dramatically decreased within 6 h after antagonist administration. These data reinforce earlier expectations that GnRH antagonists might be potential male contraceptives and provide the first report of changes in circulating bio- to immuno-FSH levels. The GAB assay will facilitate future studies on the biochemical mechanisms by which GnRH antagonists induce changes in the bioactivity of circulating FSH.

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