The cleavage sites and localization of genes encoding the restriction endonucleases Eco1831I and Ecohi

Anatoly N. Kravetz, Marina V. Zakharova, Irina V. Beletskaya, Elena V. Sineva, Marat M. Denjmuchametov, Sergey I. Petrov, Larisa I. Glatman, Alexander S. Solonin

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The restriction endonucleases Eco 1831I and Ecohi cleave before the first 5'-cytosine in the recognition sequence 5' -↓ CCSGG --3' 3' --GGSCC↑-5'(where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA+ hosts. Thus, they differ in their cleavage specificity from that of the BcnI isoschizomer, which cleaves after the second 5' cytosine.

Original languageEnglish (US)
Pages (from-to)153-154
Number of pages2
JournalGene
Volume129
Issue number1
DOIs
StatePublished - Jul 15 1993

All Science Journal Classification (ASJC) codes

  • Genetics

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