The cloning, expression and crystallisation of a thermostable arginase

Maria C. Bewley, J. Shaun Lott, Edward N. Baker, Mark L. Patchett

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The gene for the thermostable arginase from the thermophilic bacterium 'Bacillus caldovelox' has been cloned and sequenced. Expression of recombinant arginase at high levels has been achieved in E. coli using an inducible T7 RNA polymerase-based system. A facile purification procedure incorporating a heat-treatment step yielded 0.2 g of recombinant arginase per litre of induced culture. The kinetic properties of the purified recombinant protein are essentially identical to the native enzyme. The recombinant protein has been crystallised and one crystal form is isomorphous to crystals of the native protein.

Original languageEnglish (US)
Pages (from-to)215-218
Number of pages4
JournalFEBS Letters
Volume386
Issue number2-3
DOIs
StatePublished - May 20 1996

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'The cloning, expression and crystallisation of a thermostable arginase'. Together they form a unique fingerprint.

Cite this