The cloning of a rat peptidoglycan recognition protein (PGRP) and its induction in brain by sleep deprivation

Abdur Rehman, Ping Taishi, Jidong Fang, Jeannine A. Majde, James M. Krueger

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Peptidoglycan recognition protein (PGRP) binds to peptidoglycan (PG) or live bacteria and is upregulated by PG. PGRP is a ubiquitous protein involved in innate immunity. Tag7, a novel cytokine, is also induced by bacterial products; tag7 is apoptotic to murine L929 cells in a NF-κB-independent manner. Both of these genes are expressed in brain, lymphatic and haematopoietic tissues. We provide evidence that murine PGRP and tag7 encode identical transcripts and have structural relationships to lysozymes. Further, we have cloned the cDNA of rat PGRP and analyzed its expression in brains of sleep-deprived and control rats. The mRNA levels of PGRP/tag7 were measured by RT-PCR and compared to the housekeeping gene porphobilinogen deaminase (PBD). PGRP was constitutively expressed in rat brain. PGRP mRNA was increased by 43% and 17% in the brainstem and hypothalamus, respectively, in sleep-deprived rats compared to controls. The upregulation of PGRP expression by sleep deprivation suggests a role for PGRP in a homeostatic regulation of sleep.

Original languageEnglish (US)
Pages (from-to)8-17
Number of pages10
JournalCytokine
Volume13
Issue number1
DOIs
StatePublished - Jan 7 2001

Fingerprint

Sleep Deprivation
Cloning
Organism Cloning
Brain
Sleep
Peptidoglycan
Rats
Genes
Hydroxymethylbilane Synthase
Rat control
Messenger RNA
peptidoglycan recognition protein
rat Pglyrp protein
Essential Genes
Lymphoid Tissue
Muramidase
Innate Immunity
Hypothalamus
Brain Stem
Bacteria

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

Cite this

Rehman, Abdur ; Taishi, Ping ; Fang, Jidong ; Majde, Jeannine A. ; Krueger, James M. / The cloning of a rat peptidoglycan recognition protein (PGRP) and its induction in brain by sleep deprivation. In: Cytokine. 2001 ; Vol. 13, No. 1. pp. 8-17.
@article{d4d4ffc8e81e49a0bd9adf8c5cd09f9b,
title = "The cloning of a rat peptidoglycan recognition protein (PGRP) and its induction in brain by sleep deprivation",
abstract = "Peptidoglycan recognition protein (PGRP) binds to peptidoglycan (PG) or live bacteria and is upregulated by PG. PGRP is a ubiquitous protein involved in innate immunity. Tag7, a novel cytokine, is also induced by bacterial products; tag7 is apoptotic to murine L929 cells in a NF-κB-independent manner. Both of these genes are expressed in brain, lymphatic and haematopoietic tissues. We provide evidence that murine PGRP and tag7 encode identical transcripts and have structural relationships to lysozymes. Further, we have cloned the cDNA of rat PGRP and analyzed its expression in brains of sleep-deprived and control rats. The mRNA levels of PGRP/tag7 were measured by RT-PCR and compared to the housekeeping gene porphobilinogen deaminase (PBD). PGRP was constitutively expressed in rat brain. PGRP mRNA was increased by 43{\%} and 17{\%} in the brainstem and hypothalamus, respectively, in sleep-deprived rats compared to controls. The upregulation of PGRP expression by sleep deprivation suggests a role for PGRP in a homeostatic regulation of sleep.",
author = "Abdur Rehman and Ping Taishi and Jidong Fang and Majde, {Jeannine A.} and Krueger, {James M.}",
year = "2001",
month = "1",
day = "7",
doi = "10.1006/cyto.2000.0800",
language = "English (US)",
volume = "13",
pages = "8--17",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press Inc.",
number = "1",

}

The cloning of a rat peptidoglycan recognition protein (PGRP) and its induction in brain by sleep deprivation. / Rehman, Abdur; Taishi, Ping; Fang, Jidong; Majde, Jeannine A.; Krueger, James M.

In: Cytokine, Vol. 13, No. 1, 07.01.2001, p. 8-17.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The cloning of a rat peptidoglycan recognition protein (PGRP) and its induction in brain by sleep deprivation

AU - Rehman, Abdur

AU - Taishi, Ping

AU - Fang, Jidong

AU - Majde, Jeannine A.

AU - Krueger, James M.

PY - 2001/1/7

Y1 - 2001/1/7

N2 - Peptidoglycan recognition protein (PGRP) binds to peptidoglycan (PG) or live bacteria and is upregulated by PG. PGRP is a ubiquitous protein involved in innate immunity. Tag7, a novel cytokine, is also induced by bacterial products; tag7 is apoptotic to murine L929 cells in a NF-κB-independent manner. Both of these genes are expressed in brain, lymphatic and haematopoietic tissues. We provide evidence that murine PGRP and tag7 encode identical transcripts and have structural relationships to lysozymes. Further, we have cloned the cDNA of rat PGRP and analyzed its expression in brains of sleep-deprived and control rats. The mRNA levels of PGRP/tag7 were measured by RT-PCR and compared to the housekeeping gene porphobilinogen deaminase (PBD). PGRP was constitutively expressed in rat brain. PGRP mRNA was increased by 43% and 17% in the brainstem and hypothalamus, respectively, in sleep-deprived rats compared to controls. The upregulation of PGRP expression by sleep deprivation suggests a role for PGRP in a homeostatic regulation of sleep.

AB - Peptidoglycan recognition protein (PGRP) binds to peptidoglycan (PG) or live bacteria and is upregulated by PG. PGRP is a ubiquitous protein involved in innate immunity. Tag7, a novel cytokine, is also induced by bacterial products; tag7 is apoptotic to murine L929 cells in a NF-κB-independent manner. Both of these genes are expressed in brain, lymphatic and haematopoietic tissues. We provide evidence that murine PGRP and tag7 encode identical transcripts and have structural relationships to lysozymes. Further, we have cloned the cDNA of rat PGRP and analyzed its expression in brains of sleep-deprived and control rats. The mRNA levels of PGRP/tag7 were measured by RT-PCR and compared to the housekeeping gene porphobilinogen deaminase (PBD). PGRP was constitutively expressed in rat brain. PGRP mRNA was increased by 43% and 17% in the brainstem and hypothalamus, respectively, in sleep-deprived rats compared to controls. The upregulation of PGRP expression by sleep deprivation suggests a role for PGRP in a homeostatic regulation of sleep.

UR - http://www.scopus.com/inward/record.url?scp=0035819244&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035819244&partnerID=8YFLogxK

U2 - 10.1006/cyto.2000.0800

DO - 10.1006/cyto.2000.0800

M3 - Article

VL - 13

SP - 8

EP - 17

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 1

ER -