The Contribution of Chylomicron Cholesterol to Milk Cholesterol in the Rat

P. M. Kris-Etherton, Ivan D. Frantz

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The contribution of chylomicron cholesterol to total milk cholesterol was studied in the lactating rat. Pregnant rats were fed a high-fat, high-cholesterol diet or standard rat diet. On the 13th day of lactation, dams were intonated with 25 μCi [3H]cholesterol (25 μCi/μmole). Milk was collected at 5 and 10 hr following intubation; plasma was collected at 5, 8, and 10 hr following intubation. There was a lower specific activity of chylomicron cholesterol in dams fed the high-fat, high-cholesterol diet compared with control animals at 5, 8, and 10 hr after intubation (P < 0.05) with radiolabeled cholesterol. There was no difference in the specific activity of milk cholesterol between both groups at 5 hr. These data indicate that chylomicron cholesterol does not significantly contribute to milk cholesterol levels in the lactating rat. At 8 and 10 hr following intubation with radiolabeled cholesterol, the specific activity of plasma cholesterol from dams fed the high-fat, high-cholesterol diet was lower than that from control animals (P < 0.05). The specific activity of milk cholesterol at 10 hr was also lower in experimental animals (P < 0.05). Taken together, these data suggest that plasma lipoproteins other than chylomicrons contribute significantly to milk cholesterol levels.

Original languageEnglish (US)
Pages (from-to)502-507
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume165
Issue number3
DOIs
StatePublished - Dec 1980

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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