The development of affinity capture devices - A nanoscale purification platform for biological in situ transmission electron microscopy

Katherine Degen, Madeline Dukes, Justin R. Tanner, Deb Kelly

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The use of in situ transmission electron microscopy (TEM) to study biological processes is a new frontier in high-resolution imaging. Here we report the development of affinity capture devices that are used to isolate biological entities for in situ studies. Affinity capture devices are silicon nitride microchips coated with functionalized lipid monolayers. We tested the isolation capacity of the devices by using protein synthesis machinery as a test specimen for single particle and in situ TEM analysis. Overall, we demonstrate the feasibility of purifying biological assemblies in a liquid environment within a TEM column. This novel application may serve to bridge the gap between cellular and molecular imaging techniques.

Original languageEnglish (US)
Pages (from-to)2408-2412
Number of pages5
JournalRSC Advances
Volume2
Issue number6
DOIs
StatePublished - Mar 14 2012

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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