The dynamic energy landscape of dihydrofolate reductase catalysis

David D. Boehr, Dan McElheny, H. Jane Dyson, Peter E. Wrightt

Research output: Contribution to journalArticlepeer-review

699 Scopus citations

Abstract

We used nuclear magnetic resonance relaxation dispersion to characterize higher energy conformational substates of Escherichia coli dihydrofolate reductase. Each intermediate in the catalytic cycle samples low-lying excited states whose conformations resemble the ground-state structures of preceding and following intermediates. Substrate and cofactor exchange occurs through these excited substates. The maximum hydride transfer and steady-state turnover rates are governed by the dynamics of transitions between ground and excited states of the intermediates. Thus, the modulation of the energy landscape by the bound ligands funnels the enzyme through its reaction cycle along a preferred kinetic path.

Original languageEnglish (US)
Pages (from-to)1638-1642
Number of pages5
JournalScience
Volume313
Issue number5793
DOIs
StatePublished - Sep 15 2006

All Science Journal Classification (ASJC) codes

  • General

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