The effect of active-site isoleucine to alanine mutation on the DHFR catalyzed hydride-transfer

Vanja Stojković, Laura L. Perissinotti, Jeeyeon Lee, Stephen J. Benkovic, Amnon Kohen

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Comparison of the nature of hydride transfer in wild-type and active site mutant (I14A) of dihydrofolate reductase suggests that the size of this side chain at position 14 modulates H-tunneling.

Original languageEnglish (US)
Pages (from-to)8974-8976
Number of pages3
JournalChemical Communications
Volume46
Issue number47
DOIs
StatePublished - Dec 21 2010

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Tetrahydrofolate Dehydrogenase
Isoleucine
Hydrides
Alanine
Oxidoreductases

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Stojković, V., Perissinotti, L. L., Lee, J., Benkovic, S. J., & Kohen, A. (2010). The effect of active-site isoleucine to alanine mutation on the DHFR catalyzed hydride-transfer. Chemical Communications, 46(47), 8974-8976. https://doi.org/10.1039/c0cc02988b
Stojković, Vanja ; Perissinotti, Laura L. ; Lee, Jeeyeon ; Benkovic, Stephen J. ; Kohen, Amnon. / The effect of active-site isoleucine to alanine mutation on the DHFR catalyzed hydride-transfer. In: Chemical Communications. 2010 ; Vol. 46, No. 47. pp. 8974-8976.
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Stojković, V, Perissinotti, LL, Lee, J, Benkovic, SJ & Kohen, A 2010, 'The effect of active-site isoleucine to alanine mutation on the DHFR catalyzed hydride-transfer', Chemical Communications, vol. 46, no. 47, pp. 8974-8976. https://doi.org/10.1039/c0cc02988b

The effect of active-site isoleucine to alanine mutation on the DHFR catalyzed hydride-transfer. / Stojković, Vanja; Perissinotti, Laura L.; Lee, Jeeyeon; Benkovic, Stephen J.; Kohen, Amnon.

In: Chemical Communications, Vol. 46, No. 47, 21.12.2010, p. 8974-8976.

Research output: Contribution to journalArticle

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AU - Stojković, Vanja

AU - Perissinotti, Laura L.

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AU - Kohen, Amnon

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