The effect of arabinose 1,5-bisphosphate on rat hepatic 6-phosphofructo-1-kinase and fructose-1,6-bisphosphatase

Simon J. Pilkis, Molly M. McGrane, Paul D. Kountz, M. Raafat El-Maghrabi, Jo Pilkis, Bruce E. Maryanoff, Allen B. Reitz, Stephen J. Benkovic

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Abstract

The α- and β-anomers of arabinose 1,5-bisphosphate and ribose 1,5-bisphosphate were tested as effectors of rat liver 6-phosphofructo-1-kinase and fructose-1,6-bisphosphatase. Both anomers of arabinose 1,5-bisphosphate activated the kinase and inhibited the bisphosphatase. The α-anomer was the more effective kinase activator while the β-anomer was the more potent inhibitor of the bisphosphatase. Inhibition of the bisphosphatase by both anomers was competitive, and both potentiated allosteric inhibition by AMP. β-Arabinose 1,5-bisphosphate was also more effective in decreasing fructose 2,6-bisphosphate binding to the enzyme. Neither anomer of ribose 1,5-bisphosphate affected 6-phosphofructo-1-kinase or fructose-1,6-bisphosphatase, indicating that the configuration of the C-2 (C-3 in Fru 2,6-P2) hydroxyl group is important for biological activity. These results are also consistent with arabinose 1,5-bisphosphate binding to the active site and thereby enhancing the interaction of AMP with the allosteric site.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume138
Issue number1
DOIs
Publication statusPublished - Jul 16 1986

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Pilkis, S. J., McGrane, M. M., Kountz, P. D., Raafat El-Maghrabi, M., Pilkis, J., Maryanoff, B. E., ... Benkovic, S. J. (1986). The effect of arabinose 1,5-bisphosphate on rat hepatic 6-phosphofructo-1-kinase and fructose-1,6-bisphosphatase. Biochemical and Biophysical Research Communications, 138(1), 159-166. https://doi.org/10.1016/0006-291X(86)90260-3