The effects of hydralazine on arachidonic acid metabolism were studied in isolated washed human platelets. The washed platelets (10 9/ml) were exposed to 14C-arachidonic acid and incubated for 15 minutes in a platelet aggregometer (37°C). The media was then extracted and the reaction products separated by means of reversed phase high pressure liquid chromatography. In this system, hydralazine inhibited the production of TXB 2 (the stable metabolite of TXA 2) and enhanced the production of PGF 2(α) and PGE 2 in a dose dependent fashion. Indomethacin, blocked the production of all prostaglandin compounds normally synthesized by platelets. These results suggest that hydralazine is a specific thromboxane synthetase inhibitor and that its vasodilatory effects might be related to its ability to block the production of an endogenous vasoconstrictor (i.e. TXA 2).
|Original language||English (US)|
|Number of pages||10|
|Journal||Research Communications in Chemical Pathology and Pharmacology|
|State||Published - Jun 20 1980|
All Science Journal Classification (ASJC) codes
- Pathology and Forensic Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)