The effect of sheltered load on reproduction in Solanum carolinense, a species with variable self-incompatibility

Jorge I. Mena-Alí, Lidewij H. Keser, Andrew G. Stephenson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In previous studies, we have investigated the strength of self-incompatibility (SI) in Solanum carolinense, a highly successful weed with a fully functional SI system that inhabits early successional and other disturbed habitats. We have found that the SI response in S. carolinense is a plastic trait-its strength being affected by the age of the flowers, and the presence of developing fruits and that there are genetic differences among families in their self-fertility. However, in species with a fully functional SI response, selfing would not be that common. As a result, deleterious recessives scattered though the genome of horsenettle are only occasionally exposed to selection. It has been suggested that deleterious recessives accumulate near S-alleles in strong SI species because the S-locus is located in a non-recombining region of the genome and because strong S-alleles are never in the homozygous state, thus sheltering some of the genetic load near the S-locus from selection. We performed a series of laboratory and greenhouse experiments to determine the extent to which sheltered load adds to the overall magnitude of inbreeding depression in horsenettle. Specifically, we amplified and sequenced the S-alleles from 16 genets collected from a large population in Pennsylvania and performed a series of controlled self-pollinations. We then grew the selfed progeny in the greenhouse; recorded various measures of growth and reproductive output; and amplified and sequenced their S-allele(s). We found that the heterozygous progeny of self-pollinations produce more flowers and have a greater ability to set both self and cross seed than S-homozygous progeny. We also found evidence of variation in the magnitude of load among S-alleles. These results suggest that sheltered load might slow the fixation of weak (partially compatible) S-alleles in this population, thus adding to the maintenance of a mixed mating system rather than leading to the fixation of the selfing alleles.

Original languageEnglish (US)
Pages (from-to)63-71
Number of pages9
JournalSexual Plant Reproduction
Volume22
Issue number2
DOIs
StatePublished - Jun 1 2009

Fingerprint

Solanum carolinense
Solanum
Reproduction
Alleles
alleles
Pollination
self-pollination
selfing
Genetic Load
Genome
Viverridae
flowers
loci
genome
inbreeding depression
greenhouse experimentation
mating systems
Population
Plastics
Ecosystem

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Mena-Alí, Jorge I. ; Keser, Lidewij H. ; Stephenson, Andrew G. / The effect of sheltered load on reproduction in Solanum carolinense, a species with variable self-incompatibility. In: Sexual Plant Reproduction. 2009 ; Vol. 22, No. 2. pp. 63-71.
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abstract = "In previous studies, we have investigated the strength of self-incompatibility (SI) in Solanum carolinense, a highly successful weed with a fully functional SI system that inhabits early successional and other disturbed habitats. We have found that the SI response in S. carolinense is a plastic trait-its strength being affected by the age of the flowers, and the presence of developing fruits and that there are genetic differences among families in their self-fertility. However, in species with a fully functional SI response, selfing would not be that common. As a result, deleterious recessives scattered though the genome of horsenettle are only occasionally exposed to selection. It has been suggested that deleterious recessives accumulate near S-alleles in strong SI species because the S-locus is located in a non-recombining region of the genome and because strong S-alleles are never in the homozygous state, thus sheltering some of the genetic load near the S-locus from selection. We performed a series of laboratory and greenhouse experiments to determine the extent to which sheltered load adds to the overall magnitude of inbreeding depression in horsenettle. Specifically, we amplified and sequenced the S-alleles from 16 genets collected from a large population in Pennsylvania and performed a series of controlled self-pollinations. We then grew the selfed progeny in the greenhouse; recorded various measures of growth and reproductive output; and amplified and sequenced their S-allele(s). We found that the heterozygous progeny of self-pollinations produce more flowers and have a greater ability to set both self and cross seed than S-homozygous progeny. We also found evidence of variation in the magnitude of load among S-alleles. These results suggest that sheltered load might slow the fixation of weak (partially compatible) S-alleles in this population, thus adding to the maintenance of a mixed mating system rather than leading to the fixation of the selfing alleles.",
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The effect of sheltered load on reproduction in Solanum carolinense, a species with variable self-incompatibility. / Mena-Alí, Jorge I.; Keser, Lidewij H.; Stephenson, Andrew G.

In: Sexual Plant Reproduction, Vol. 22, No. 2, 01.06.2009, p. 63-71.

Research output: Contribution to journalArticle

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