The effect of transmembrane potential on the gating of MSCL channels in droplet interface bilayers

Joseph S. Najem, Sergei Sukharev, Donald J. Leo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The ability to functionalize droplet interface bilayers (DIBs) with the MscL channel and its mutants has been demonstrated. In previous work, the V23T gain of function mutant of MscL produced consistent activation when harmonic axial compressions were applied to the aqueous droplets supporting the lipid bilayer, where the channels settle. The deformation of the droplets results, at maximum compression, in an increase in surface area, and thus an increase in tension at the water-lipid-oil interface. This increase in monolayer tension was found to be the product of the relative change in surface area of each of the droplets and the compressibility modulus of the DPhPC monolayer (∼120 mN/m). The tension increase at the water-lipid-oil interface almost doubles to make up the increase in tension in the bilayer interface, resulting in activation of the incorporated MscL channels. However, it was found that the application of a relatively high transmembrane potential (∼100 mV), from an external power source, is a requirement for the activation of the V23T-MscL channels. Here, we investigate and analyze the impact of transmembrane potential on the activity of MscL channels in both a droplet interface bilayer system and E. coli spheroplast via patch-clamp. We demonstrate that the channels became more susceptible to gating upon the application of a negative potential, compared to when a positive potential is applied, proving their sensitivity to voltage polarity.

Original languageEnglish (US)
Title of host publicationModeling, Simulation and Control; Bio-Inspired Smart Materials and Systems; Energy Harvesting
PublisherAmerican Society of Mechanical Engineers
ISBN (Electronic)9780791850497
DOIs
StatePublished - Jan 1 2016
EventASME 2016 Conference on Smart Materials, Adaptive Structures and Intelligent Systems, SMASIS 2016 - Stowe, United States
Duration: Sep 28 2016Sep 30 2016

Publication series

NameASME 2016 Conference on Smart Materials, Adaptive Structures and Intelligent Systems, SMASIS 2016
Volume2

Other

OtherASME 2016 Conference on Smart Materials, Adaptive Structures and Intelligent Systems, SMASIS 2016
CountryUnited States
CityStowe
Period9/28/169/30/16

All Science Journal Classification (ASJC) codes

  • Building and Construction
  • Civil and Structural Engineering
  • Control and Systems Engineering
  • Mechanics of Materials

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