The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri

Wolfram M. Brück, Shannon Kelleher, Glenn R. Gibson, Gitte Graverholt, Bo L. Lönnerdal

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Two milk components, α-lactalbumin (α-La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. 3[H] thymidine-labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo-2 cultures and their association with CaCo-2 cells was assessed. Undigested, pepsin-digested and pepsin- and pancreatin-digested α-lactalbumin and glycomacropeptide inhibited association. Thus, milk supplemented with α-lactalbumin and glycomacropeptide might be effective in inhibiting associations of the pathogens EPEC, Salmonella typhimurium, and Shigella flexneri to intestinal cells.

Original languageEnglish (US)
Pages (from-to)158-162
Number of pages5
JournalFEMS Microbiology Letters
Volume259
Issue number1
DOIs
StatePublished - Jun 1 2006

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Shigella flexneri
Enteropathogenic Escherichia coli
Lactalbumin
Caco-2 Cells
Salmonella
Pepsin A
Salmonella typhimurium
Milk
Pancreatin
Thymidine
Infection
caseinomacropeptide

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Brück, Wolfram M. ; Kelleher, Shannon ; Gibson, Glenn R. ; Graverholt, Gitte ; Lönnerdal, Bo L. / The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri. In: FEMS Microbiology Letters. 2006 ; Vol. 259, No. 1. pp. 158-162.
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abstract = "Two milk components, α-lactalbumin (α-La) and glycomacropeptide (GMP) may inhibit intestinal infection/intoxification. 3[H] thymidine-labeled enteropathogenic Escherichia coli (EPEC), Salmonella typhimurium (ATCC 6994) or Shigella flexneri (ATCC 9199) were introduced to CaCo-2 cultures and their association with CaCo-2 cells was assessed. Undigested, pepsin-digested and pepsin- and pancreatin-digested α-lactalbumin and glycomacropeptide inhibited association. Thus, milk supplemented with α-lactalbumin and glycomacropeptide might be effective in inhibiting associations of the pathogens EPEC, Salmonella typhimurium, and Shigella flexneri to intestinal cells.",
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The effects of α-lactalbumin and glycomacropeptide on the association of CaCo-2 cells by enteropathogenic Escherichia coli, Salmonella typhimuriumand Shigella flexneri. / Brück, Wolfram M.; Kelleher, Shannon; Gibson, Glenn R.; Graverholt, Gitte; Lönnerdal, Bo L.

In: FEMS Microbiology Letters, Vol. 259, No. 1, 01.06.2006, p. 158-162.

Research output: Contribution to journalArticle

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