The end adjusts the means: Heterochromatin remodelling during terminal cell differentiation

Sergei A. Grigoryev, Yaroslava A. Bulynko, Evgenya Y. Popova

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

All cells that constitute mature tissues in an eukaryotic organism undergo a multistep process of cell differentiation. At the terminal stage of this process, cells either cease to proliferate forever or rest for a very long period of time. During terminal differentiation, most of the genes that are required for cell 'housekeeping' functions, such as proto-oncogenes and other cell-cycle and cell proliferation genes, become stably repressed. At the same time, nuclear chromatin undergoes dramatic morphological and structural changes at the higher-order levels of chromatin organization. These changes involve both constitutively inactive chromosomal regions (constitutive heterochromatin) and the formerly active genes that become silenced and structurally modified to form facultative heterochromatin. Here we approach terminal cell differentiation as a unique system that allows us to combine biochemical, ultrastructural and molecular genetic techniques to study the relationship between the hierarchy of chromatin higher-order structures in the nucleus and its function(s) in dynamic packing of genetic material in a form that remains amenable to regulation of gene activity and other DNA-dependent cellular processes.

Original languageEnglish (US)
Pages (from-to)53-69
Number of pages17
JournalChromosome Research
Volume14
Issue number1
DOIs
StatePublished - Feb 1 2006

Fingerprint

Heterochromatin
Cell Differentiation
Chromatin
Genes
Molecular Biology
Genetic Techniques
Housekeeping
Proto-Oncogenes
Cell Cycle
Cell Proliferation
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

@article{2a0ea7c314414123aa9ca4a841a70c16,
title = "The end adjusts the means: Heterochromatin remodelling during terminal cell differentiation",
abstract = "All cells that constitute mature tissues in an eukaryotic organism undergo a multistep process of cell differentiation. At the terminal stage of this process, cells either cease to proliferate forever or rest for a very long period of time. During terminal differentiation, most of the genes that are required for cell 'housekeeping' functions, such as proto-oncogenes and other cell-cycle and cell proliferation genes, become stably repressed. At the same time, nuclear chromatin undergoes dramatic morphological and structural changes at the higher-order levels of chromatin organization. These changes involve both constitutively inactive chromosomal regions (constitutive heterochromatin) and the formerly active genes that become silenced and structurally modified to form facultative heterochromatin. Here we approach terminal cell differentiation as a unique system that allows us to combine biochemical, ultrastructural and molecular genetic techniques to study the relationship between the hierarchy of chromatin higher-order structures in the nucleus and its function(s) in dynamic packing of genetic material in a form that remains amenable to regulation of gene activity and other DNA-dependent cellular processes.",
author = "Grigoryev, {Sergei A.} and Bulynko, {Yaroslava A.} and Popova, {Evgenya Y.}",
year = "2006",
month = "2",
day = "1",
doi = "10.1007/s10577-005-1021-6",
language = "English (US)",
volume = "14",
pages = "53--69",
journal = "Chromosome Research",
issn = "0967-3849",
publisher = "Springer Netherlands",
number = "1",

}

The end adjusts the means : Heterochromatin remodelling during terminal cell differentiation. / Grigoryev, Sergei A.; Bulynko, Yaroslava A.; Popova, Evgenya Y.

In: Chromosome Research, Vol. 14, No. 1, 01.02.2006, p. 53-69.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The end adjusts the means

T2 - Heterochromatin remodelling during terminal cell differentiation

AU - Grigoryev, Sergei A.

AU - Bulynko, Yaroslava A.

AU - Popova, Evgenya Y.

PY - 2006/2/1

Y1 - 2006/2/1

N2 - All cells that constitute mature tissues in an eukaryotic organism undergo a multistep process of cell differentiation. At the terminal stage of this process, cells either cease to proliferate forever or rest for a very long period of time. During terminal differentiation, most of the genes that are required for cell 'housekeeping' functions, such as proto-oncogenes and other cell-cycle and cell proliferation genes, become stably repressed. At the same time, nuclear chromatin undergoes dramatic morphological and structural changes at the higher-order levels of chromatin organization. These changes involve both constitutively inactive chromosomal regions (constitutive heterochromatin) and the formerly active genes that become silenced and structurally modified to form facultative heterochromatin. Here we approach terminal cell differentiation as a unique system that allows us to combine biochemical, ultrastructural and molecular genetic techniques to study the relationship between the hierarchy of chromatin higher-order structures in the nucleus and its function(s) in dynamic packing of genetic material in a form that remains amenable to regulation of gene activity and other DNA-dependent cellular processes.

AB - All cells that constitute mature tissues in an eukaryotic organism undergo a multistep process of cell differentiation. At the terminal stage of this process, cells either cease to proliferate forever or rest for a very long period of time. During terminal differentiation, most of the genes that are required for cell 'housekeeping' functions, such as proto-oncogenes and other cell-cycle and cell proliferation genes, become stably repressed. At the same time, nuclear chromatin undergoes dramatic morphological and structural changes at the higher-order levels of chromatin organization. These changes involve both constitutively inactive chromosomal regions (constitutive heterochromatin) and the formerly active genes that become silenced and structurally modified to form facultative heterochromatin. Here we approach terminal cell differentiation as a unique system that allows us to combine biochemical, ultrastructural and molecular genetic techniques to study the relationship between the hierarchy of chromatin higher-order structures in the nucleus and its function(s) in dynamic packing of genetic material in a form that remains amenable to regulation of gene activity and other DNA-dependent cellular processes.

UR - http://www.scopus.com/inward/record.url?scp=33644631504&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644631504&partnerID=8YFLogxK

U2 - 10.1007/s10577-005-1021-6

DO - 10.1007/s10577-005-1021-6

M3 - Article

C2 - 16506096

AN - SCOPUS:33644631504

VL - 14

SP - 53

EP - 69

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 1

ER -