The enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro

Kristin L. Shingler, Javier O. Cifuente, Robert E. Ashley, Alexander M. Makhov, James F. Conway, Susan Hafenstein

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Enterovirus 71 (EV71) is responsible for seasonal outbreaks of hand, foot, and mouth disease in the Asia-Pacific region. The virus has the capability to cause severe disease and death, especially in young children. Although several vaccines are currently in clinical trials, no vaccines or therapeutics have been approved for use. Previous structural studies have revealed that two antigenically distinct capsid forms are produced in EV71-infected cells: an expanded empty capsid, sometimes called a procapsid, and the infectious virus. Specifically, an immunodominant epitope of EV71 that maps to the virus canyon is structurally different in the procapsid and virus. This structure-function study shows that the procapsid can sequester antibodies, thus enhancing EV71 infection in vitro. The results presented here suggest that, due to conformational differences between the EV71 procapsid and virus, the presence of the procapsid in natural virus infections should be considered in the future design of vaccines or therapeutics.

Original languageEnglish (US)
Pages (from-to)1900-1908
Number of pages9
JournalJournal of virology
Volume89
Issue number3
DOIs
StatePublished - Jan 1 2015

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Enterovirus
Virus Diseases
Neutralizing Antibodies
neutralizing antibodies
Viruses
viruses
infection
Vaccines
Capsid
capsid
vaccines
Hand, Foot and Mouth Disease
Enterovirus Infections
Immunodominant Epitopes
hand, foot and mouth disease
Blocking Antibodies
therapeutics
canyons
Disease Outbreaks
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Shingler, Kristin L. ; Cifuente, Javier O. ; Ashley, Robert E. ; Makhov, Alexander M. ; Conway, James F. ; Hafenstein, Susan. / The enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro. In: Journal of virology. 2015 ; Vol. 89, No. 3. pp. 1900-1908.
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The enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro. / Shingler, Kristin L.; Cifuente, Javier O.; Ashley, Robert E.; Makhov, Alexander M.; Conway, James F.; Hafenstein, Susan.

In: Journal of virology, Vol. 89, No. 3, 01.01.2015, p. 1900-1908.

Research output: Contribution to journalArticle

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