The essential polysome-associated RNA-binding protein RBP42 targets mRNAs involved in Trypanosoma brucei energy metabolism

Anish Das, Rachel Morales, Mahrukh Banday, Stacey Garcia, Li Hao, George A.M. Cross, Antonio M. Estevez, Vivian Bellofatto

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

RNA-binding proteins that target mRNA coding regions are emerging as regulators of post-transcriptional processes in eukaryotes. Here we describe a newly identified RNA-binding protein, RBP42, which targets the coding region of mRNAs in the insect form of the African trypanosome, Trypanosoma brucei. RBP42 is an essential protein and associates with polysome-bound mRNAs in the cytoplasm. A global survey of RBP42-bound mRNAs was performed by applying HITS-CLIP technology, which captures protein-RNA interactions in vivo using UV light. Specific RBP42-mRNA interactions, as well as mRNA interactions with a known RNA-binding protein, were purified using specific antibodies. Target RNA sequences were identified and quantified using high-throughput RNA sequencing. Analysis revealed that RBP42 bound mainly within the coding region of mRNAs that encode proteins involved in cellular energy metabolism. Although the mechanism of RBP42's function is unclear at present, we speculate that RBP42 plays a critical role in modulating T. brucei energy metabolism. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1968-1983
Number of pages16
JournalRNA
Volume18
Issue number11
DOIs
StatePublished - Nov 1 2012

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Trypanosoma brucei brucei
RNA-Binding Proteins
Polyribosomes
Energy Metabolism
Messenger RNA
High-Throughput Nucleotide Sequencing
Proteins
Trypanosomiasis
Ultraviolet Rays
Eukaryota
Insects
Cytoplasm
RNA
Technology
Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Das, Anish ; Morales, Rachel ; Banday, Mahrukh ; Garcia, Stacey ; Hao, Li ; Cross, George A.M. ; Estevez, Antonio M. ; Bellofatto, Vivian. / The essential polysome-associated RNA-binding protein RBP42 targets mRNAs involved in Trypanosoma brucei energy metabolism. In: RNA. 2012 ; Vol. 18, No. 11. pp. 1968-1983.
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abstract = "RNA-binding proteins that target mRNA coding regions are emerging as regulators of post-transcriptional processes in eukaryotes. Here we describe a newly identified RNA-binding protein, RBP42, which targets the coding region of mRNAs in the insect form of the African trypanosome, Trypanosoma brucei. RBP42 is an essential protein and associates with polysome-bound mRNAs in the cytoplasm. A global survey of RBP42-bound mRNAs was performed by applying HITS-CLIP technology, which captures protein-RNA interactions in vivo using UV light. Specific RBP42-mRNA interactions, as well as mRNA interactions with a known RNA-binding protein, were purified using specific antibodies. Target RNA sequences were identified and quantified using high-throughput RNA sequencing. Analysis revealed that RBP42 bound mainly within the coding region of mRNAs that encode proteins involved in cellular energy metabolism. Although the mechanism of RBP42's function is unclear at present, we speculate that RBP42 plays a critical role in modulating T. brucei energy metabolism. Published by Cold Spring Harbor Laboratory Press.",
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Das, A, Morales, R, Banday, M, Garcia, S, Hao, L, Cross, GAM, Estevez, AM & Bellofatto, V 2012, 'The essential polysome-associated RNA-binding protein RBP42 targets mRNAs involved in Trypanosoma brucei energy metabolism', RNA, vol. 18, no. 11, pp. 1968-1983. https://doi.org/10.1261/rna.033829.112

The essential polysome-associated RNA-binding protein RBP42 targets mRNAs involved in Trypanosoma brucei energy metabolism. / Das, Anish; Morales, Rachel; Banday, Mahrukh; Garcia, Stacey; Hao, Li; Cross, George A.M.; Estevez, Antonio M.; Bellofatto, Vivian.

In: RNA, Vol. 18, No. 11, 01.11.2012, p. 1968-1983.

Research output: Contribution to journalArticle

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AU - Cross, George A.M.

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