The evidence for clonal spreading of quinolone resistance with a particular clonal complex of Campylobacter jejuni

J. Kovač, N. Čadež, M. Lušicky, E. Møller Nielsen, M. Ocepek, P. Raspor, S. Smole Možina

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Campylobacter is the most prevalent cause of bacterial gastroenteritis worldwide and it represents a significant public health risk of increasing severity due to its escalating resistance to clinically important quinolone and macrolide antibiotics. As a zoonotic pathogen Campylobacter is transmitted along the food chain and naturally cycles from environmental waters, feedstuff, animals and food to humans. We determined antibiotic resistance profiles, as well as multilocus sequence types and flaA-SVR types for 52 C. jejuni isolated in Slovenia from human, animal, raw and cured chicken meat and water samples. Twenty-eight different sequence types, arranged in ten clonal complexes, three new allele types and five new sequence types were identified, indicating the relatively high diversity in a small group of strains. The assignment of strains from different sources to the same clonal complexes indicates their transmission along the food supply chain. The most prevalent clonal complex was CC21, which was also the genetic group with 95% of quinolone-resistant strains. Based on the genetic relatedness of these quinolone-resistant strains identified by polymerase chain reaction with a mismatch amplification mutation assay and sequencing of the quinolone resistance-determining region of the gyrA gene, we conclude that the high resistance prevalence observed indicates the local clonal spread of quinolone resistance with CC21.

Original languageEnglish (US)
Pages (from-to)2595-2603
Number of pages9
JournalEpidemiology and Infection
Volume142
Issue number12
DOIs
StatePublished - Dec 14 2014

Fingerprint

Campylobacter jejuni
Quinolones
Campylobacter
Food Chain
Water Cycle
Slovenia
Food Supply
Macrolides
Zoonoses
Gastroenteritis
Microbial Drug Resistance
Meat
Chickens
Public Health
Alleles
Anti-Bacterial Agents
Food
Polymerase Chain Reaction
Mutation
Water

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Infectious Diseases

Cite this

Kovač, J. ; Čadež, N. ; Lušicky, M. ; Nielsen, E. Møller ; Ocepek, M. ; Raspor, P. ; Smole Možina, S. / The evidence for clonal spreading of quinolone resistance with a particular clonal complex of Campylobacter jejuni. In: Epidemiology and Infection. 2014 ; Vol. 142, No. 12. pp. 2595-2603.
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The evidence for clonal spreading of quinolone resistance with a particular clonal complex of Campylobacter jejuni. / Kovač, J.; Čadež, N.; Lušicky, M.; Nielsen, E. Møller; Ocepek, M.; Raspor, P.; Smole Možina, S.

In: Epidemiology and Infection, Vol. 142, No. 12, 14.12.2014, p. 2595-2603.

Research output: Contribution to journalArticle

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