The expression of alternative oxidase and alternative respiratory capacity in cytoplasmic male sterile common bean

C. Johns, R. Nickels, L. McIntosh, Sally Mackenzie

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The alternative respiratory pathway is present in all plant species investigated to date. Yet, the role of the alternative pathway is not clear. Some evidence suggests an important role in pollen development. We undertook this study to investigate the expression of alternative oxidase, in comparison with expression of a component of cytochrome oxidase, during pollen formation in common bean (Phaseolus vulgaris L.). Expression was evaluated immunohistochemically. In addition, we compared both the alternative oxidase capacity in young seedling tissues and alternative oxidase expression in developing flower buds of isonuclear cytoplasmic male sterile and male fertile bean lines. We observed no evidence of an association between the abnormal pollen development of CMS bean and changes in alternative oxidase expression or capacity. We did observe a tissueand stage-specific pattern of expression of alternative oxidase, differing from the expression pattern of cytochrome oxidase subunit II, during anther development in normal bean lines. Although no association was evident between the cytoplasmic male sterility phenotype and differential expression of alternative oxidase, the regulated pattern of alternative oxidase expression in developing anthers does suggest that the alternative pathway may play a role in microgametogenesis and microsporogenesis.

Original languageEnglish (US)
Pages (from-to)257-265
Number of pages9
JournalSexual Plant Reproduction
Volume6
Issue number4
DOIs
StatePublished - Oct 1 1993

Fingerprint

beans
pollen
cytochrome-c oxidase
anthers
Pollen
microsporogenesis
cytoplasmic male sterility
Electron Transport Complex IV
Phaseolus vulgaris
Plant Gametogenesis
buds
Phaseolus
phenotype
Male Infertility
seedlings
alternative oxidase
Seedlings
Phenotype

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

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The expression of alternative oxidase and alternative respiratory capacity in cytoplasmic male sterile common bean. / Johns, C.; Nickels, R.; McIntosh, L.; Mackenzie, Sally.

In: Sexual Plant Reproduction, Vol. 6, No. 4, 01.10.1993, p. 257-265.

Research output: Contribution to journalArticle

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AU - Nickels, R.

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AU - Mackenzie, Sally

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AB - The alternative respiratory pathway is present in all plant species investigated to date. Yet, the role of the alternative pathway is not clear. Some evidence suggests an important role in pollen development. We undertook this study to investigate the expression of alternative oxidase, in comparison with expression of a component of cytochrome oxidase, during pollen formation in common bean (Phaseolus vulgaris L.). Expression was evaluated immunohistochemically. In addition, we compared both the alternative oxidase capacity in young seedling tissues and alternative oxidase expression in developing flower buds of isonuclear cytoplasmic male sterile and male fertile bean lines. We observed no evidence of an association between the abnormal pollen development of CMS bean and changes in alternative oxidase expression or capacity. We did observe a tissueand stage-specific pattern of expression of alternative oxidase, differing from the expression pattern of cytochrome oxidase subunit II, during anther development in normal bean lines. Although no association was evident between the cytoplasmic male sterility phenotype and differential expression of alternative oxidase, the regulated pattern of alternative oxidase expression in developing anthers does suggest that the alternative pathway may play a role in microgametogenesis and microsporogenesis.

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