The expression of insecticide resistance-related cytochrome p450 forms is regulated by molting hormone in Drosophila melanogaster

Vladimir Spiegelman, Serge Y. Fuchs, Gennady A. Belitsky

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The expression and enzymatic activities of insecticide resistance-related cytochrome P450B are increased by the treatment with 20-hydroxyecdysone (20HE) in D. melanogaster Oregon R flies. We have explored the role of this hormone in the maintenance of P450B basal expression. Arrest of ecdysone synthesis led to a decrease in CYP6A2 mRNA level, as well as in P450B expression and activities. This effect occurred both in insecticide susceptible (ecd1) and resistant (IRED) strains carrying the temperature-sensitive ecd mutation. The role of the 20HE in the regulation of cytochrome P450-mediated insecticide resistance has been proposed.

Original languageEnglish (US)
Pages (from-to)304-307
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume232
Issue number2
DOIs
StatePublished - Mar 17 1997

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Insecticide Resistance
Ecdysterone
Ecdysone
Insecticides
Drosophila melanogaster
Cytochrome P-450 Enzyme System
Cytochromes
Diptera
Maintenance
Hormones
Messenger RNA
Mutation
Temperature

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "The expression and enzymatic activities of insecticide resistance-related cytochrome P450B are increased by the treatment with 20-hydroxyecdysone (20HE) in D. melanogaster Oregon R flies. We have explored the role of this hormone in the maintenance of P450B basal expression. Arrest of ecdysone synthesis led to a decrease in CYP6A2 mRNA level, as well as in P450B expression and activities. This effect occurred both in insecticide susceptible (ecd1) and resistant (IRED) strains carrying the temperature-sensitive ecd mutation. The role of the 20HE in the regulation of cytochrome P450-mediated insecticide resistance has been proposed.",
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The expression of insecticide resistance-related cytochrome p450 forms is regulated by molting hormone in Drosophila melanogaster. / Spiegelman, Vladimir; Fuchs, Serge Y.; Belitsky, Gennady A.

In: Biochemical and Biophysical Research Communications, Vol. 232, No. 2, 17.03.1997, p. 304-307.

Research output: Contribution to journalArticle

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