The fragile fiber1 kinesin contributes to cortical microtubule-mediated trafficking of cell wall components

Chuanmei Zhu, Anindya Ganguly, Tobias I. Baskin, Daniel D. McClosky, Charles T. Anderson, Cliff Foster, Kristoffer A. Meunier, Ruth Okamoto, Howard Berg, Ram Dixit

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The cell wall consists of cellulose microfibrils embedded within a matrix of hemicellulose and pectin. Cellulose microfibrils are synthesized at the plasma membrane, whereas matrix polysaccharides are synthesized in the Golgi apparatus and secreted. The trafficking of vesicles containing cell wall components is thought to depend on actin-myosin. Here, we implicate microtubules in this process through studies of the kinesin-4 family member, Fragile Fiber1 (FRA1). In an fra1-5 knockout mutant, the expansion rate of the inflorescence stem is halved compared with the wild type along with the thickness of both primary and secondary cell walls. Nevertheless, cell walls in fra1-5 have an essentially unaltered composition and ultrastructure. A functional triple green fluorescent protein-tagged FRA1 fusion protein moves processively along cortical microtubules, and its abundance and motile density correlate with growth rate. Motility of FRA1 and cellulose synthase complexes is independent, indicating that FRA1 is not directly involved in cellulose biosynthesis; however, the secretion rate of fucose-alkyne-labeled pectin is greatly decreased in fra1-5, and the mutant has Golgi bodies with fewer cisternae and enlarged vesicles. Based on our results, we propose that FRA1 contributes to cell wall production by transporting Golgi-derived vesicles along cortical microtubules for secretion.

Original languageEnglish (US)
Pages (from-to)780-792
Number of pages13
JournalPlant physiology
Volume167
Issue number3
DOIs
StatePublished - Jan 1 2015

Fingerprint

Kinesin
kinesin
cell wall components
Cellular Structures
Microtubules
Cell Wall
microtubules
cell walls
cellulose
Cellulose
Microfibrils
Golgi apparatus
pectins
alkynes
secretion
cellulose synthase
knockout mutants
fucose
myosin
Inflorescence

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

Zhu, Chuanmei ; Ganguly, Anindya ; Baskin, Tobias I. ; McClosky, Daniel D. ; Anderson, Charles T. ; Foster, Cliff ; Meunier, Kristoffer A. ; Okamoto, Ruth ; Berg, Howard ; Dixit, Ram. / The fragile fiber1 kinesin contributes to cortical microtubule-mediated trafficking of cell wall components. In: Plant physiology. 2015 ; Vol. 167, No. 3. pp. 780-792.
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Zhu, C, Ganguly, A, Baskin, TI, McClosky, DD, Anderson, CT, Foster, C, Meunier, KA, Okamoto, R, Berg, H & Dixit, R 2015, 'The fragile fiber1 kinesin contributes to cortical microtubule-mediated trafficking of cell wall components', Plant physiology, vol. 167, no. 3, pp. 780-792. https://doi.org/10.1104/pp.114.251462

The fragile fiber1 kinesin contributes to cortical microtubule-mediated trafficking of cell wall components. / Zhu, Chuanmei; Ganguly, Anindya; Baskin, Tobias I.; McClosky, Daniel D.; Anderson, Charles T.; Foster, Cliff; Meunier, Kristoffer A.; Okamoto, Ruth; Berg, Howard; Dixit, Ram.

In: Plant physiology, Vol. 167, No. 3, 01.01.2015, p. 780-792.

Research output: Contribution to journalArticle

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