The genome of M. acetivorans reveals extensive metabolic and physiological diversity

James E. Galagan, Chad Nusbaum, Alice Roy, Matthew G. Endrizzi, Pendexter Macdonald, Will Fitzhugh, Sarah Calvo, Reinhard Engels, Serge Smirnov, Deven Atnoor, Adam Brown, Nicole Allen, Jerome Naylor, Nicole Stange-Thomann, Kurt Dearellano, Robin Johnson, Lauren Linton, Paul McEwan, Kevin McKernan, Jessica TalamasAndrea Tirrell, Wenjuan Ye, Andrew Zimmer, Robert D. Barber, Isaac Cann, David E. Graham, David A. Grahame, Adam M. Guss, Reiner Hedderich, Cheryl Ingram-Smith, H. Craig Kuettner, Joseph A. Krzycki, John A. Leigh, Weixi Li, Jinfeng Liu, Biswarup Mukhopadhyay, John N. Reeve, Kerry Smith, Timothy A. Springer, Lowell A. Umayam, Owen White, Robert H. White, Everly Conway De Macario, James G. Ferry, Ken F. Jarrell, Hua Jing, Alberto J.L. Macario, Ian Paulsen, Matthew Pritchett, Kevin R. Sowers, Ronald V. Swanson, Steven H. Zinder, Eric Lander, William W. Metcalf, Bruce Birren

Research output: Contribution to journalArticle

434 Scopus citations

Abstract

Methanogenesis, the biological production of methane, plays a pivotal role in the global carbon cycle and contributes significantly to global warming. The majority of methane in nature is derived from acetate. Here we report the complete genome sequence of an acetate-utilizing methanogen, Methanosarcina acetivorans C2A. Methanosarcineae are the most metabolically diverse methanogens, thrive in a broad range of environments, and are unique among the Archaea in forming complex multicellular structures. This diversity is reflected in the genome of M. acetivorans. At 5,751,492 base pairs it is by far the largest known archaeal genome. The 4524 open reading frames code for a strikingly wide and unanticipated variety of metabolic and cellular capabilities. The presence of novel methyltransferases indicates the likelihood of undiscovered natural energy sources for methanogenesis, whereas the presence of single-subunit carbon monoxide dehydrogenases raises the possibility of nonmethanogenic growth. Although motility has not been observed in any Methanosarcineae, a flagellin gene cluster and two complete chemotaxis gene clusters were identified. The availability of genetic methods, coupled with its physiological and metabolic diversity, makes M. acetivorans a powerful model organism for the study of archaeal biology.

Original languageEnglish (US)
Pages (from-to)532-542
Number of pages11
JournalGenome research
Volume12
Issue number4
DOIs
StatePublished - Apr 25 2002

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

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    Galagan, J. E., Nusbaum, C., Roy, A., Endrizzi, M. G., Macdonald, P., Fitzhugh, W., Calvo, S., Engels, R., Smirnov, S., Atnoor, D., Brown, A., Allen, N., Naylor, J., Stange-Thomann, N., Dearellano, K., Johnson, R., Linton, L., McEwan, P., McKernan, K., ... Birren, B. (2002). The genome of M. acetivorans reveals extensive metabolic and physiological diversity. Genome research, 12(4), 532-542. https://doi.org/10.1101/gr.223902