The heparan sulfate proteoglycans Dally-like and Syndecan have distinct functions in axon guidance and visual-system assembly in Drosophila

Joel M. Rawson, Brian Dimitroff, Karl G. Johnson, Jaime M. Rawson, Xuecai Ge, David Van Vactor, Scott Brian Selleck

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Heparan sulfate proteoglycans (HSPGs), a class of glycosaminoglycan- modified proteins, control diverse patterning events via their regulation of growth-factor signaling and morphogen distribution [1]. In C. elegans, zebrafish, and the mouse, heparan sulfate (HS) biosynthesis is required for normal axon guidance [2-4], and mutations affecting Syndecan (Sdc), a transmembrane HSPG, disrupt axon guidance in Drosophila embryos [5, 6]. Glypicans, a family of glycosylphosphatidylinositol (GPI)-linked HSPGs, are expressed on axons and growth cones in vertebrates, but their role in axon guidance has not been determined [7, 8]. We demonstrate here that the Drosophila glypican Dally-like protein (Dlp) is required for proper axon guidance and visual-system function. Mosaic studies revealed that Dlp is necessary in both the retina and the brain for different aspects of visual-system assembly. Sdc mutants also showed axon guidance and visual-system defects, some that overlap with dlp and others that are unique. dlp+ transgenes were able to rescue some sdc visual-system phenotypes, but sdc+ transgenes were ineffective in rescuing dlp abnormalities. Together, these findings suggest that in some contexts HS chains provide the biologically critical component, whereas in others the structure of the protein core is also essential.

Original languageEnglish (US)
Pages (from-to)833-838
Number of pages6
JournalCurrent Biology
Volume15
Issue number9
DOIs
StatePublished - May 10 2005

Fingerprint

Syndecans
Heparan Sulfate Proteoglycans
proteoglycans
axons
Drosophila
Glypicans
Heparitin Sulfate
Transgenes
transgenes
Proteins
Glycosylphosphatidylinositols
Growth Cones
Zebrafish
Glycosaminoglycans
proteins
cones (retina)
Biosynthesis
glycosaminoglycans
Axons
protein structure

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Rawson, Joel M. ; Dimitroff, Brian ; Johnson, Karl G. ; Rawson, Jaime M. ; Ge, Xuecai ; Van Vactor, David ; Selleck, Scott Brian. / The heparan sulfate proteoglycans Dally-like and Syndecan have distinct functions in axon guidance and visual-system assembly in Drosophila. In: Current Biology. 2005 ; Vol. 15, No. 9. pp. 833-838.
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The heparan sulfate proteoglycans Dally-like and Syndecan have distinct functions in axon guidance and visual-system assembly in Drosophila. / Rawson, Joel M.; Dimitroff, Brian; Johnson, Karl G.; Rawson, Jaime M.; Ge, Xuecai; Van Vactor, David; Selleck, Scott Brian.

In: Current Biology, Vol. 15, No. 9, 10.05.2005, p. 833-838.

Research output: Contribution to journalArticle

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