The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17

Atsushi Ogo, Michael R. Waterman, Janette McAllister, Norio Kagawa

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Pbx1 is a homeodomain transcription factor involved in cAMP-dependent transcriptional regulation of the bovine CYP17 gene. In this study, we have investigated the involvement of Pbx1 in the transcriptional regulation of the human CYP17 gene. Although a sequence identical to previously determined Pbx- binding sites is not present in the promoter region of the human CYP17 gene, three putative Pbx-binding sites are identified by sequence similarity analysis. Coexpression of Pbx1 and a catalytic subunit of protein kinase A (PKA) greatly enhances reporter gene transcription via the 5'-flanking region of the human CYP17 gene. Upon gel shift analysis utilizing nuclear extracts from human adrenal H295R cells, one of the three putative Pbx1-binding sites, -250/-241 bp, shows the typical intense doublet observed with other Pbx- binding sites. 5'-Deletion analyses of the reporter construct containing this Pbx-binding site showed approximately sixfold induction by coexpression of Pbx1 and PKA compared to the basal transcription, suggesting that Pbx1 binds the -250/ -241 bp sequence and participates in cAMP-dependent regulation of the human CYP17 gene.

Original languageEnglish (US)
Pages (from-to)226-231
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume348
Issue number1
DOIs
StatePublished - Dec 1 1997

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

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