The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17

Atsushi Ogo, Michael R. Waterman, Janette McAllister, Norio Kagawa

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Pbx1 is a homeodomain transcription factor involved in cAMP-dependent transcriptional regulation of the bovine CYP17 gene. In this study, we have investigated the involvement of Pbx1 in the transcriptional regulation of the human CYP17 gene. Although a sequence identical to previously determined Pbx- binding sites is not present in the promoter region of the human CYP17 gene, three putative Pbx-binding sites are identified by sequence similarity analysis. Coexpression of Pbx1 and a catalytic subunit of protein kinase A (PKA) greatly enhances reporter gene transcription via the 5'-flanking region of the human CYP17 gene. Upon gel shift analysis utilizing nuclear extracts from human adrenal H295R cells, one of the three putative Pbx1-binding sites, -250/-241 bp, shows the typical intense doublet observed with other Pbx- binding sites. 5'-Deletion analyses of the reporter construct containing this Pbx-binding site showed approximately sixfold induction by coexpression of Pbx1 and PKA compared to the basal transcription, suggesting that Pbx1 binds the -250/ -241 bp sequence and participates in cAMP-dependent regulation of the human CYP17 gene.

Original languageEnglish (US)
Pages (from-to)226-231
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume348
Issue number1
DOIs
StatePublished - Dec 1 1997

Fingerprint

Steroid 17-alpha-Hydroxylase
Homeodomain Proteins
Transcription
Genes
Binding Sites
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
5' Flanking Region
Electrophoretic Mobility Shift Assay
Cyclic AMP-Dependent Protein Kinases
Reporter Genes
Genetic Promoter Regions
Sequence Analysis
Transcription Factors
Gels

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Ogo, Atsushi ; Waterman, Michael R. ; McAllister, Janette ; Kagawa, Norio. / The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17. In: Archives of Biochemistry and Biophysics. 1997 ; Vol. 348, No. 1. pp. 226-231.
@article{e8cbb387c92f410597f4ab1564d97aa1,
title = "The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17",
abstract = "Pbx1 is a homeodomain transcription factor involved in cAMP-dependent transcriptional regulation of the bovine CYP17 gene. In this study, we have investigated the involvement of Pbx1 in the transcriptional regulation of the human CYP17 gene. Although a sequence identical to previously determined Pbx- binding sites is not present in the promoter region of the human CYP17 gene, three putative Pbx-binding sites are identified by sequence similarity analysis. Coexpression of Pbx1 and a catalytic subunit of protein kinase A (PKA) greatly enhances reporter gene transcription via the 5'-flanking region of the human CYP17 gene. Upon gel shift analysis utilizing nuclear extracts from human adrenal H295R cells, one of the three putative Pbx1-binding sites, -250/-241 bp, shows the typical intense doublet observed with other Pbx- binding sites. 5'-Deletion analyses of the reporter construct containing this Pbx-binding site showed approximately sixfold induction by coexpression of Pbx1 and PKA compared to the basal transcription, suggesting that Pbx1 binds the -250/ -241 bp sequence and participates in cAMP-dependent regulation of the human CYP17 gene.",
author = "Atsushi Ogo and Waterman, {Michael R.} and Janette McAllister and Norio Kagawa",
year = "1997",
month = "12",
day = "1",
doi = "10.1006/abbi.1997.0372",
language = "English (US)",
volume = "348",
pages = "226--231",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Academic Press Inc.",
number = "1",

}

The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17. / Ogo, Atsushi; Waterman, Michael R.; McAllister, Janette; Kagawa, Norio.

In: Archives of Biochemistry and Biophysics, Vol. 348, No. 1, 01.12.1997, p. 226-231.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The homeodomain protein Pbx1 is involved in cAMP-dependent transcription of human CYP17

AU - Ogo, Atsushi

AU - Waterman, Michael R.

AU - McAllister, Janette

AU - Kagawa, Norio

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Pbx1 is a homeodomain transcription factor involved in cAMP-dependent transcriptional regulation of the bovine CYP17 gene. In this study, we have investigated the involvement of Pbx1 in the transcriptional regulation of the human CYP17 gene. Although a sequence identical to previously determined Pbx- binding sites is not present in the promoter region of the human CYP17 gene, three putative Pbx-binding sites are identified by sequence similarity analysis. Coexpression of Pbx1 and a catalytic subunit of protein kinase A (PKA) greatly enhances reporter gene transcription via the 5'-flanking region of the human CYP17 gene. Upon gel shift analysis utilizing nuclear extracts from human adrenal H295R cells, one of the three putative Pbx1-binding sites, -250/-241 bp, shows the typical intense doublet observed with other Pbx- binding sites. 5'-Deletion analyses of the reporter construct containing this Pbx-binding site showed approximately sixfold induction by coexpression of Pbx1 and PKA compared to the basal transcription, suggesting that Pbx1 binds the -250/ -241 bp sequence and participates in cAMP-dependent regulation of the human CYP17 gene.

AB - Pbx1 is a homeodomain transcription factor involved in cAMP-dependent transcriptional regulation of the bovine CYP17 gene. In this study, we have investigated the involvement of Pbx1 in the transcriptional regulation of the human CYP17 gene. Although a sequence identical to previously determined Pbx- binding sites is not present in the promoter region of the human CYP17 gene, three putative Pbx-binding sites are identified by sequence similarity analysis. Coexpression of Pbx1 and a catalytic subunit of protein kinase A (PKA) greatly enhances reporter gene transcription via the 5'-flanking region of the human CYP17 gene. Upon gel shift analysis utilizing nuclear extracts from human adrenal H295R cells, one of the three putative Pbx1-binding sites, -250/-241 bp, shows the typical intense doublet observed with other Pbx- binding sites. 5'-Deletion analyses of the reporter construct containing this Pbx-binding site showed approximately sixfold induction by coexpression of Pbx1 and PKA compared to the basal transcription, suggesting that Pbx1 binds the -250/ -241 bp sequence and participates in cAMP-dependent regulation of the human CYP17 gene.

UR - http://www.scopus.com/inward/record.url?scp=0031543341&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031543341&partnerID=8YFLogxK

U2 - 10.1006/abbi.1997.0372

DO - 10.1006/abbi.1997.0372

M3 - Article

C2 - 9390195

AN - SCOPUS:0031543341

VL - 348

SP - 226

EP - 231

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 1

ER -