The human gut microbiome as a screening tool for colorectal cancer

Joseph P. Zackular, Mary A.M. Rogers, Mack Ruffin, Patrick D. Schloss

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Recent studies have suggested that the gut microbiome may be an important factor in the development of colorectal cancer. Abnormalities in the gut microbiome have been reported in patients with colorectal cancer; however, this microbial community has not been explored as a potential screen for early-stage disease. We characterized the gut microbiome in patients from three clinical groups representing the stages of colorectal cancer development: healthy, adenoma, and carcinoma. Analysis of the gut microbiome from stool samples revealed both an enrichment and depletion of several bacterial populations associated with adenomas and carcinomas. Combined with known clinical risk factors of colorectal cancer (e.g., BMI, age, race), data fromthe gut microbiome significantly improved the ability to differentiate between healthy, adenoma, and carcinoma clinical groups relative to risk factors alone. Using Bayesian methods, we determined that using gut microbiome data as a screening tool improved the pretest to posttest probability of adenomamore than 50-fold. For example, the pretest probability in a 65-year-old was 0.17% and, after using the microbiome data, this increased to 10.67% (1 in 9 chance of having an adenoma). Taken together, the results of our study demonstrate the feasibility of using the composition of the gut microbiome to detect the presence of precancerous and cancerous lesions. Furthermore, these results support the need for more cross-sectional studies with diverse populations and linkage to other stool markers, dietary data, and personal health information.

Original languageEnglish (US)
Pages (from-to)1112-1121
Number of pages10
JournalCancer Prevention Research
Volume7
Issue number11
DOIs
StatePublished - Nov 1 2014

Fingerprint

Microbiota
Colorectal Neoplasms
Adenoma
Carcinoma
Personal Health Records
Bayes Theorem
Feasibility Studies
Gastrointestinal Microbiome
Population
Cross-Sectional Studies

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Zackular, Joseph P. ; Rogers, Mary A.M. ; Ruffin, Mack ; Schloss, Patrick D. / The human gut microbiome as a screening tool for colorectal cancer. In: Cancer Prevention Research. 2014 ; Vol. 7, No. 11. pp. 1112-1121.
@article{34d16a1266ac45798698492e13869449,
title = "The human gut microbiome as a screening tool for colorectal cancer",
abstract = "Recent studies have suggested that the gut microbiome may be an important factor in the development of colorectal cancer. Abnormalities in the gut microbiome have been reported in patients with colorectal cancer; however, this microbial community has not been explored as a potential screen for early-stage disease. We characterized the gut microbiome in patients from three clinical groups representing the stages of colorectal cancer development: healthy, adenoma, and carcinoma. Analysis of the gut microbiome from stool samples revealed both an enrichment and depletion of several bacterial populations associated with adenomas and carcinomas. Combined with known clinical risk factors of colorectal cancer (e.g., BMI, age, race), data fromthe gut microbiome significantly improved the ability to differentiate between healthy, adenoma, and carcinoma clinical groups relative to risk factors alone. Using Bayesian methods, we determined that using gut microbiome data as a screening tool improved the pretest to posttest probability of adenomamore than 50-fold. For example, the pretest probability in a 65-year-old was 0.17{\%} and, after using the microbiome data, this increased to 10.67{\%} (1 in 9 chance of having an adenoma). Taken together, the results of our study demonstrate the feasibility of using the composition of the gut microbiome to detect the presence of precancerous and cancerous lesions. Furthermore, these results support the need for more cross-sectional studies with diverse populations and linkage to other stool markers, dietary data, and personal health information.",
author = "Zackular, {Joseph P.} and Rogers, {Mary A.M.} and Mack Ruffin and Schloss, {Patrick D.}",
year = "2014",
month = "11",
day = "1",
doi = "10.1158/1940-6207.CAPR-14-0129",
language = "English (US)",
volume = "7",
pages = "1112--1121",
journal = "Cancer Prevention Research",
issn = "1940-6207",
publisher = "American Association for Cancer Research Inc.",
number = "11",

}

The human gut microbiome as a screening tool for colorectal cancer. / Zackular, Joseph P.; Rogers, Mary A.M.; Ruffin, Mack; Schloss, Patrick D.

In: Cancer Prevention Research, Vol. 7, No. 11, 01.11.2014, p. 1112-1121.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The human gut microbiome as a screening tool for colorectal cancer

AU - Zackular, Joseph P.

AU - Rogers, Mary A.M.

AU - Ruffin, Mack

AU - Schloss, Patrick D.

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Recent studies have suggested that the gut microbiome may be an important factor in the development of colorectal cancer. Abnormalities in the gut microbiome have been reported in patients with colorectal cancer; however, this microbial community has not been explored as a potential screen for early-stage disease. We characterized the gut microbiome in patients from three clinical groups representing the stages of colorectal cancer development: healthy, adenoma, and carcinoma. Analysis of the gut microbiome from stool samples revealed both an enrichment and depletion of several bacterial populations associated with adenomas and carcinomas. Combined with known clinical risk factors of colorectal cancer (e.g., BMI, age, race), data fromthe gut microbiome significantly improved the ability to differentiate between healthy, adenoma, and carcinoma clinical groups relative to risk factors alone. Using Bayesian methods, we determined that using gut microbiome data as a screening tool improved the pretest to posttest probability of adenomamore than 50-fold. For example, the pretest probability in a 65-year-old was 0.17% and, after using the microbiome data, this increased to 10.67% (1 in 9 chance of having an adenoma). Taken together, the results of our study demonstrate the feasibility of using the composition of the gut microbiome to detect the presence of precancerous and cancerous lesions. Furthermore, these results support the need for more cross-sectional studies with diverse populations and linkage to other stool markers, dietary data, and personal health information.

AB - Recent studies have suggested that the gut microbiome may be an important factor in the development of colorectal cancer. Abnormalities in the gut microbiome have been reported in patients with colorectal cancer; however, this microbial community has not been explored as a potential screen for early-stage disease. We characterized the gut microbiome in patients from three clinical groups representing the stages of colorectal cancer development: healthy, adenoma, and carcinoma. Analysis of the gut microbiome from stool samples revealed both an enrichment and depletion of several bacterial populations associated with adenomas and carcinomas. Combined with known clinical risk factors of colorectal cancer (e.g., BMI, age, race), data fromthe gut microbiome significantly improved the ability to differentiate between healthy, adenoma, and carcinoma clinical groups relative to risk factors alone. Using Bayesian methods, we determined that using gut microbiome data as a screening tool improved the pretest to posttest probability of adenomamore than 50-fold. For example, the pretest probability in a 65-year-old was 0.17% and, after using the microbiome data, this increased to 10.67% (1 in 9 chance of having an adenoma). Taken together, the results of our study demonstrate the feasibility of using the composition of the gut microbiome to detect the presence of precancerous and cancerous lesions. Furthermore, these results support the need for more cross-sectional studies with diverse populations and linkage to other stool markers, dietary data, and personal health information.

UR - http://www.scopus.com/inward/record.url?scp=84910116215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84910116215&partnerID=8YFLogxK

U2 - 10.1158/1940-6207.CAPR-14-0129

DO - 10.1158/1940-6207.CAPR-14-0129

M3 - Article

C2 - 25104642

AN - SCOPUS:84910116215

VL - 7

SP - 1112

EP - 1121

JO - Cancer Prevention Research

JF - Cancer Prevention Research

SN - 1940-6207

IS - 11

ER -