The human secretome atlas initiative

Implications in health and disease conditions

Kristy J. Brown, Haeri Seol, Dinesh K. Pillai, Binu-john Sankoorikal, Catherine A. Formolo, Jenny Mac, Nathan J. Edwards, Mary C. Rose, Yetrib Hathout

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Proteomic analysis of human body fluids is highly challenging, therefore many researchers are redirecting efforts toward secretome profiling. The goal is to define potential biomarkers and therapeutic targets in the secretome that can be traced back in accessible human body fluids. However, currently there is a lack of secretome profiles of normal human primary cells making it difficult to assess the biological meaning of current findings. In this study we sought to establish secretome profiles of human primary cells obtained from healthy donors with the goal of building a human secretome atlas. Such an atlas can be used as a reference for discovery of potential disease associated biomarkers and eventually novel therapeutic targets. As a preliminary study, secretome profiles were established for six different types of human primary cell cultures and checked for overlaps with the three major human body fluids including plasma, cerebrospinal fluid and urine. About 67% of the 1054 identified proteins in the secretome of these primary cells occurred in at least one body fluid. Furthermore, comparison of the secretome profiles of two human glioblastoma cell lines to this new human secretome atlas enabled unambiguous identification of potential brain tumor biomarkers. These biomarkers can be easily monitored in different body fluids using stable isotope labeled standard proteins. The long term goal of this study is to establish a comprehensive online human secretome atlas for future use as a reference for any disease related secretome study. This article is part of a Special Issue entitled: An Updated Secretome.

Original languageEnglish (US)
Pages (from-to)2454-2461
Number of pages8
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1834
Issue number11
DOIs
StatePublished - Oct 1 2013

Fingerprint

Atlases
Body fluids
Body Fluids
Health
Biomarkers
Human Body
Cerebrospinal fluid
Tumor Biomarkers
Cell culture
Isotopes
Primary Cell Culture
Brain
Proteins
Glioblastoma
Cells
Brain Neoplasms
Proteomics
Plasmas
Cerebrospinal Fluid
Research Personnel

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Brown, Kristy J. ; Seol, Haeri ; Pillai, Dinesh K. ; Sankoorikal, Binu-john ; Formolo, Catherine A. ; Mac, Jenny ; Edwards, Nathan J. ; Rose, Mary C. ; Hathout, Yetrib. / The human secretome atlas initiative : Implications in health and disease conditions. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2013 ; Vol. 1834, No. 11. pp. 2454-2461.
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Brown, KJ, Seol, H, Pillai, DK, Sankoorikal, B, Formolo, CA, Mac, J, Edwards, NJ, Rose, MC & Hathout, Y 2013, 'The human secretome atlas initiative: Implications in health and disease conditions', Biochimica et Biophysica Acta - Proteins and Proteomics, vol. 1834, no. 11, pp. 2454-2461. https://doi.org/10.1016/j.bbapap.2013.04.007

The human secretome atlas initiative : Implications in health and disease conditions. / Brown, Kristy J.; Seol, Haeri; Pillai, Dinesh K.; Sankoorikal, Binu-john; Formolo, Catherine A.; Mac, Jenny; Edwards, Nathan J.; Rose, Mary C.; Hathout, Yetrib.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1834, No. 11, 01.10.2013, p. 2454-2461.

Research output: Contribution to journalArticle

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