The immature and the mature myocardium. Responses to multidose crystalloid cardioplegia

J. A. Magovern, Walter Pae, C. A. Miller, J. A. Waldhausen

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This study was designed to determine whether multidose St. Thomas' Hospital cardioplegic solution is as effective for preservation of the immature myocardium during ischemia as it is for the mature myocardium. An isolated working heart model was used. Sets of six hearts from immature (3 to 4 weeks, 500 gm) and mature (24 weeks, 2 kg) rabbits were subjected to 60, 90, or 120 minutes of ischemia. Myocardial protection consisted of infusion of cardioplegic solution every 30 minutes at 4°C in a dose of 10 ml/kg of animal weight and maintenance of hypothermia at 10°C by immersion in a cold saline bath. The percent recovery of preischemic aortic flow was lower in the immature than the mature hearts after 90 minutes (60.3% ± 7.4% versus 101.8% ± 4.3%) and after 120 minutes (57.4% ± 10.6% versus 91.1% ± 13.6%) of ischemia (results expressed as mean value ± standard error of the mean, p < 0.05). There were no differences between the mature and the immature hearts in the recovery of heart rate, left atrial pressure, mean aortic pressure, or glycogen stores. Adenosine tiphosphate levels measured at the end of the experiment were not different from control in the immature hearts subjected to 60 or 90 minutes of ischemia, but did decline after 120 minutes of ischemia (18.5 ± 0.8, 16.9 ± 1.3, 16.6 ± 0.6 versus 12.3 ± 1.8 μmol/gm dry weight, p <0.05). Adenosine triphosphate levels in the mature hearts were lower than control in hearts subjected to 60, 90, and 120 minutes of ischemia (18.0 ± 1.2 versus 13.6 ± 1.1, 12.8 ± 0.9, 13.7 ± 1.5 μmol/gm dry weight, p <0.05). Multidose St. Thomas' Hospital cardioplegia does not provide adequate preservation of hemodynamic function in the immature rabbit heart, even though myocardial high-energy spores are well preserved. Additional work is necessary to clarify the mechanism of this finding and to develop appropriate methods for protection of the immature myocardium.

Original languageEnglish (US)
Pages (from-to)618-624
Number of pages7
JournalJournal of Thoracic and Cardiovascular Surgery
Volume95
Issue number4
StatePublished - Jan 1 1988

Fingerprint

Induced Heart Arrest
Myocardium
Ischemia
Weights and Measures
Cardioplegic Solutions
Rabbits
Atrial Pressure
crystalloid solutions
Immersion
Hypothermia
Spores
Glycogen
Baths
Adenosine
Arterial Pressure
Adenosine Triphosphate
Heart Rate
Hemodynamics
Maintenance

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

@article{71bbf62cbd44443aab1e13fc0785b0a0,
title = "The immature and the mature myocardium. Responses to multidose crystalloid cardioplegia",
abstract = "This study was designed to determine whether multidose St. Thomas' Hospital cardioplegic solution is as effective for preservation of the immature myocardium during ischemia as it is for the mature myocardium. An isolated working heart model was used. Sets of six hearts from immature (3 to 4 weeks, 500 gm) and mature (24 weeks, 2 kg) rabbits were subjected to 60, 90, or 120 minutes of ischemia. Myocardial protection consisted of infusion of cardioplegic solution every 30 minutes at 4°C in a dose of 10 ml/kg of animal weight and maintenance of hypothermia at 10°C by immersion in a cold saline bath. The percent recovery of preischemic aortic flow was lower in the immature than the mature hearts after 90 minutes (60.3{\%} ± 7.4{\%} versus 101.8{\%} ± 4.3{\%}) and after 120 minutes (57.4{\%} ± 10.6{\%} versus 91.1{\%} ± 13.6{\%}) of ischemia (results expressed as mean value ± standard error of the mean, p < 0.05). There were no differences between the mature and the immature hearts in the recovery of heart rate, left atrial pressure, mean aortic pressure, or glycogen stores. Adenosine tiphosphate levels measured at the end of the experiment were not different from control in the immature hearts subjected to 60 or 90 minutes of ischemia, but did decline after 120 minutes of ischemia (18.5 ± 0.8, 16.9 ± 1.3, 16.6 ± 0.6 versus 12.3 ± 1.8 μmol/gm dry weight, p <0.05). Adenosine triphosphate levels in the mature hearts were lower than control in hearts subjected to 60, 90, and 120 minutes of ischemia (18.0 ± 1.2 versus 13.6 ± 1.1, 12.8 ± 0.9, 13.7 ± 1.5 μmol/gm dry weight, p <0.05). Multidose St. Thomas' Hospital cardioplegia does not provide adequate preservation of hemodynamic function in the immature rabbit heart, even though myocardial high-energy spores are well preserved. Additional work is necessary to clarify the mechanism of this finding and to develop appropriate methods for protection of the immature myocardium.",
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The immature and the mature myocardium. Responses to multidose crystalloid cardioplegia. / Magovern, J. A.; Pae, Walter; Miller, C. A.; Waldhausen, J. A.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 95, No. 4, 01.01.1988, p. 618-624.

Research output: Contribution to journalArticle

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N2 - This study was designed to determine whether multidose St. Thomas' Hospital cardioplegic solution is as effective for preservation of the immature myocardium during ischemia as it is for the mature myocardium. An isolated working heart model was used. Sets of six hearts from immature (3 to 4 weeks, 500 gm) and mature (24 weeks, 2 kg) rabbits were subjected to 60, 90, or 120 minutes of ischemia. Myocardial protection consisted of infusion of cardioplegic solution every 30 minutes at 4°C in a dose of 10 ml/kg of animal weight and maintenance of hypothermia at 10°C by immersion in a cold saline bath. The percent recovery of preischemic aortic flow was lower in the immature than the mature hearts after 90 minutes (60.3% ± 7.4% versus 101.8% ± 4.3%) and after 120 minutes (57.4% ± 10.6% versus 91.1% ± 13.6%) of ischemia (results expressed as mean value ± standard error of the mean, p < 0.05). There were no differences between the mature and the immature hearts in the recovery of heart rate, left atrial pressure, mean aortic pressure, or glycogen stores. Adenosine tiphosphate levels measured at the end of the experiment were not different from control in the immature hearts subjected to 60 or 90 minutes of ischemia, but did decline after 120 minutes of ischemia (18.5 ± 0.8, 16.9 ± 1.3, 16.6 ± 0.6 versus 12.3 ± 1.8 μmol/gm dry weight, p <0.05). Adenosine triphosphate levels in the mature hearts were lower than control in hearts subjected to 60, 90, and 120 minutes of ischemia (18.0 ± 1.2 versus 13.6 ± 1.1, 12.8 ± 0.9, 13.7 ± 1.5 μmol/gm dry weight, p <0.05). Multidose St. Thomas' Hospital cardioplegia does not provide adequate preservation of hemodynamic function in the immature rabbit heart, even though myocardial high-energy spores are well preserved. Additional work is necessary to clarify the mechanism of this finding and to develop appropriate methods for protection of the immature myocardium.

AB - This study was designed to determine whether multidose St. Thomas' Hospital cardioplegic solution is as effective for preservation of the immature myocardium during ischemia as it is for the mature myocardium. An isolated working heart model was used. Sets of six hearts from immature (3 to 4 weeks, 500 gm) and mature (24 weeks, 2 kg) rabbits were subjected to 60, 90, or 120 minutes of ischemia. Myocardial protection consisted of infusion of cardioplegic solution every 30 minutes at 4°C in a dose of 10 ml/kg of animal weight and maintenance of hypothermia at 10°C by immersion in a cold saline bath. The percent recovery of preischemic aortic flow was lower in the immature than the mature hearts after 90 minutes (60.3% ± 7.4% versus 101.8% ± 4.3%) and after 120 minutes (57.4% ± 10.6% versus 91.1% ± 13.6%) of ischemia (results expressed as mean value ± standard error of the mean, p < 0.05). There were no differences between the mature and the immature hearts in the recovery of heart rate, left atrial pressure, mean aortic pressure, or glycogen stores. Adenosine tiphosphate levels measured at the end of the experiment were not different from control in the immature hearts subjected to 60 or 90 minutes of ischemia, but did decline after 120 minutes of ischemia (18.5 ± 0.8, 16.9 ± 1.3, 16.6 ± 0.6 versus 12.3 ± 1.8 μmol/gm dry weight, p <0.05). Adenosine triphosphate levels in the mature hearts were lower than control in hearts subjected to 60, 90, and 120 minutes of ischemia (18.0 ± 1.2 versus 13.6 ± 1.1, 12.8 ± 0.9, 13.7 ± 1.5 μmol/gm dry weight, p <0.05). Multidose St. Thomas' Hospital cardioplegia does not provide adequate preservation of hemodynamic function in the immature rabbit heart, even though myocardial high-energy spores are well preserved. Additional work is necessary to clarify the mechanism of this finding and to develop appropriate methods for protection of the immature myocardium.

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